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Ein Assay zur Messung der Aktivität von Escherichia coli Inducible Lysin Decarboxyase

Article DOI: 10.3791/2094-v • 08:09 min • December 19th, 2010
December 19th, 2010 •
1, 1
1Department of Biochemistry, University of Toronto

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Die Aktivität der induzierbaren Lysin-Decarboxylase wird durch Umsetzung des Substrates L-Lysin und das Produkt Cadaverin mit 2,4,6-trinitrobenzensulfonic Säure-Addukte, die unterschiedliche Löslichkeit in Toluol Form überwacht.

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Biochemie induzierbare Lysin Decarboxyase Acid Stress Strenge Response Pyridoxal-5'-Phosphat-abhängige Decarboxylase Guanosin-5'-diphosphat 3'-diphosphat
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