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Bioengineering

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Stretching korta sekvenser av DNA med konstant kraft Axial optisk pincett

Article DOI: 10.3791/3405-v • 08:48 min • October 13th, 2011
October 13th, 2011 •
1, 2, 2
1LSA Biophysics, University of Michigan, 2LSA Biophysics, Department of Physics, University of Michigan

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Vi illustrerar användningen av en konstant kraft axiell optisk pincett att utforska de mekaniska egenskaperna hos korta DNA-molekyler. Genom att sträcka DNA axiellt, minimerar vi steriska hinder och artefakter som uppstår vid konventionell lateral manipulation, vilket tillåter oss att studera DNA-molekyler så korta som ~ 100 nm.

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Bioteknik genetik DNA stretching DNA axiella optisk pincett enda molekyl biofysik biofysik
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