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En kvantitativ analys för att studera protein
Screening for Functional Non-coding Genetic Variants Using Electrophoretic Mobility Shift Assay (EMSA) and DNA-affinity Precipitation Assay (DAPA)
Journal (Biology)
Screening for Functional Non-coding Genetic Variants Using Electrophoretic Mobility Shift Assay (EMSA) and DNA-affinity Precipitation Assay (DAPA)
Automating Aggregate Quantification in Caenorhabditis elegans
Journal (Biology)
Automating Aggregate Quantification in Caenorhabditis elegans
 
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En kvantitativ analys för att studera protein: DNA-interaktioner, Discover transkriptionsregulatorer av genuttryck, och identifiera nya antitumörmedel

Article DOI: 10.3791/50512-v • 06:43 min • August 31st, 2013
August 31st, 2013 •
1, 1, 2, 3, 4, 3,5
1Greenebaum Cancer Center, University of Maryland School of Medicine, 2Program in Molecular Medicine, University of Maryland School of Medicine, 3Department of Biochemistry & Molecular Biology, University of Maryland School of Medicine, 4Department of Pharmacology & Experimental Therapeutics, University of Maryland School of Medicine, 5Department of Pathology and Biochemistry & Molecular Biology, University of Maryland School of Medicine

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Vi utvecklade en kvantitativ DNA-bindning ELISA-baserad analys för att mäta transkriptionsfaktor interaktion med DNA. Hög specificitet för Runx2 protein uppnåddes med en konsensus-DNA-erkännande oligonukleotiden och specifik monoklonal antikropp. Kolorimetrisk detektion med en enzym-kopplad antikropp substratreaktionen övervakades i realtid.

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Cellulär Biology transkriptionsfaktorer vitamin D Drug Discovery enzymkopplad immunadsorberande analys (ELISA) DNA-bindande transkriptionsfaktor läkemedelsscreening antikropp
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