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JoVE Journal Bioengineering
Biosensores radiométricos que miden el estado redox mitocondrial y ATP en las células vivas de levadura
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Biosensores radiométricos que miden el estado redox mitocondrial y ATP en las células vivas de levadura

Article DOI: 10.3791/50633-v • 12:22 min • July 22nd, 2013
July 22nd, 2013 •
*1, *1, 2, 2, 1
1Department of Pathology and Cell Biology, Columbia University, 2Herbert Irving Comprehensive Cancer Center, Columbia University
* These authors contributed equally

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Se describe el uso de dos, biosensores ratiometric codificados genéticamente, que se basan en la GFP, para vigilar los niveles de ATP estado redox mitocondrial y en la resolución subcelular en células de levadura vivas.

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Bioingeniería Número 77 Microbiología Biología Celular Biología Molecular Bioquímica Ciencias de la Vida roGFP la proteína verde fluorescente sensible a redox GO-ATeam ATP FRET ROS las mitocondrias los biosensores GFP ImageJ microscopía microscopía confocal celular formación de imágenes
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