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Quantifizierung der Brustkrebs-Zelle Invasivität Mit einem Drei-dimensionale (3D) Modell
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Quantifizierung der Brustkrebs-Zelle Invasivität Mit einem Drei-dimensionale (3D) Modell

Article DOI: 10.3791/51341-v • 08:08 min • June 11th, 2014
June 11th, 2014 •
*1, *1, 1,2,3
1Department of Physiology and Pharmacology, Schulich School of Medicine and Dentistry, University of Western Ontario, 2Department of Oncology, Schulich School of Medicine and Dentistry, University of Western Ontario, 3Lawson Health Research Institute
* These authors contributed equally

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Dieser Artikel enthält detaillierte Methoden für die Verwendung von dreidimensionalen (3D-) Assays auf Brustkrebszellinvasion zu quantifizieren. Insbesondere diskutieren wir die Verfahren erforderlich, um solche Tests einrichten, Quantifizierung und Datenanalyse, sowie Methoden, um den Verlust der Membranintegrität, die bei Zellen eindringen tritt zu untersuchen.

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Medizin Brustkrebs Zellinvasion extrazellulären Matrix (ECM) drei-dimensionale (3D-) Kulturen Immunzytochemie Matrigel Basalmembranmatrix
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