Rivista
/
Medicina
/
Quantification of Breast Cancer Cell Invasiveness Using a Three-dimensional (3D) Model
JoVE Journal
Medicina
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Medicina
Quantification of Breast Cancer Cell Invasiveness Using a Three-dimensional (3D) Model

Quantification of Breast Cancer Cell Invasiveness Using a Three-dimensional (3D) Model

15,654 Views

•

08:08 min

•

June 11, 2014

DOI:

10.3791/51341-v

DOI:
10.3791/51341-v

•

08:08 min
June 11, 2014

•

5 Views
, ,
1Department of Physiology and Pharmacology, Schulich School of Medicine and Dentistry,University of Western Ontario, 2Department of Oncology, Schulich School of Medicine and Dentistry,University of Western Ontario, 3Lawson Health Research Institute

Summary

Automatically generated

This article provides detailed methodologies for the use of three-dimensional (3D) assays to quantify breast cancer cell invasion. Specifically, we discuss the procedures required to set up such assays, quantification, and data analysis, as well as methods to examine the loss of membrane integrity that occurs when cells invade.

Explore More Videos

Breast Cancer3D Cell CultureExtracellular MatrixInvasionMorphologyImmunostaining

Read Article

Cite this Article

Cvetković, D., Goertzen, C. G., Bhattacharya, M. Quantification of Breast Cancer Cell Invasiveness Using a Three-dimensional (3D) Model. J. Vis. Exp. (88), e51341, doi:10.3791/51341 (2014).

Download .ris file

Copy

Share Video

.

Copy