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La combinaison unique molécule manipulation et d'imagerie pour l'étude des interactions protéine-ADN
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La combinaison unique molécule manipulation et d'imagerie pour l'étude des interactions protéine-ADN

Article DOI: 10.3791/51446-v • 14:43 min • August 27th, 2014
August 27th, 2014 •
1,2, 1, 1,3, 1,4,5,6, 1,4
1LENS - European Laboratory for Non-linear Spectroscopy, University of Florence, 2Chemistry Research Laboratory, University of Oxford, 3Department of Biology, University of Florence, 4Department of Physics and Astronomy, University of Florence, 5National Institute of Optics-National Research Council, Italy, 6International Center of Computational Neurophotonics

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Nous décrivons ici l'instrumentation et des méthodes pour la détection de molécules de protéine unique marqués par fluorescence qui interagissent avec une molécule d'ADN unique suspendu entre deux microsphères optiquement piégés.

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Bio-ingénierie Numéro 90 la biophysique des molécules simples pinces optiques microscopie à fluorescence les protéines de liaison d'ADN répresseur lactose la microfluidique
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