Un<em> Ex Vivo</em> Laser-indotta Spinal Cord Injury modello per valutare i meccanismi di degenerazione assonale in tempo reale
10,854 Views
•
11:18 min
•
November 25, 2014
DOI:
Summary
Automatically generated
We present a protocol utilizing two-photon excitation time-lapse microscopy to simultaneously visualize the dynamics of axon and myelin injuries in real time. This proposed protocol permits studies of both intrinsic and extrinsic factors which can influence central myelinated axon fate after injury and contribute to permanent clinical disability.
Video correlati
10:24
In vivo Imaging of the Mouse Spinal Cord Using Two-photon Microscopy
Video correlati
23953 Views
06:39
Live Imaging of Dorsal Root Axons after Rhizotomy
Video correlati
13822 Views
09:25
Laser Nanosurgery of Cerebellar Axons In Vivo
Video correlati
8185 Views
11:20
A Procedure for Implanting a Spinal Chamber for Longitudinal In Vivo Imaging of the Mouse Spinal Cord
Video correlati
14138 Views
08:43
Intravital Imaging of Axonal Interactions with Microglia and Macrophages in a Mouse Dorsal Column Crush Injury
Video correlati
11457 Views
08:27
Photothrombosis-induced Focal Ischemia as a Model of Spinal Cord Injury in Mice
Video correlati
14107 Views
09:25
An In Vivo Duo-color Method for Imaging Vascular Dynamics Following Contusive Spinal Cord Injury
Video correlati
9420 Views
09:55
A Drosophila In Vivo Injury Model for Studying Neuroregeneration in the Peripheral and Central Nervous System
Video correlati
9689 Views
10:27
Real-Time Assessment of Spinal Cord Microperfusion in a Porcine Model of Ischemia/Reperfusion
Video correlati
3457 Views
09:43
Controlled Semi-Automated Laser-Induced Injuries for Studying Spinal Cord Regeneration in Zebrafish Larvae
Video correlati
2322 Views
Read Article
Cite this Article
Okada, S. L. M., Stivers, N. S., Stys, P. K., Stirling, D. P. An Ex Vivo Laser-induced Spinal Cord Injury Model to Assess Mechanisms of Axonal Degeneration in Real-time. J. Vis. Exp. (93), e52173, doi:10.3791/52173 (2014).
Copy