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Analysis of Gene Expression Changes in the Rat Hippocampus After Deep Brain Stimulation of the Anterior Thalamic Nucleus

Article DOI: 10.3791/52457-v • 09:46 min • March 8th, 2015
March 8th, 2015 •
1, 2, 1
1Department of Neurosurgery, Brigham & Women's Hospital, Harvard Medical School, 2Division of Brain Sciences, Department of Medicine, Imperial College London

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The mechanism underlying the therapeutic effects of Deep Brain Stimulation (DBS) surgery needs investigation. The methods presented in this manuscript describe an experimental approach to examine the cellular events triggered by DBS by analyzing the gene expression profile of candidate genes that can facilitate neurogenesis post DBS surgery.

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Gene Expression Changes Rat Hippocampus Deep Brain Stimulation Anterior Thalamic Nucleus DBS Surgery Movement Disorders Medication Basal Ganglia Thalamus Subthalamic Regions Morbid Obesity Depression Obsessive Compulsive Disorder Physiological Mechanisms Gene Expression Analysis Neurogenesis Rat Dentate Gyrus Transcriptional Changes Electrically Stimulating ATN Stereotactically-guided DBS Surgery Adult Male Wistar Rats Tissue Dissection RNA Isolation CDNA Preparation Quantitative RT-PCR
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