Automated Quantification of Hematopoietic Cell &#8211; Stromal Cell Interactions in Histological Images of Undecalcified Bone

Sandra Zehentmeier; Zoltan Cseresnyes; Juan Escribano Navarro; Raluca A. Niesner; Anja E. Hauser

doi:10.3791/52544

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Automated quantificazione di cellule ematopoietiche - Interazioni cellule stromali in istologici Immagini di Undecalcified Bone

Automated Quantification of Hematopoietic Cell – Stromal Cell Interactions in Histological Images of Undecalcified Bone

JoVE Journal
Biologia dello sviluppo

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JoVE Journal Biologia dello sviluppo

Automated Quantification of Hematopoietic Cell – Stromal Cell Interactions in Histological Images of Undecalcified Bone

Automated quantificazione di cellule ematopoietiche - Interazioni cellule stromali in istologici Immagini di Undecalcified Bone

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11,551 Views

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09:31 min

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April 08, 2015

DOI:

10.3791/52544-v

DOI:

10.3791/52544-v

•

09:31 min

April 08, 2015

•

11526 Views

Sandra Zehentmeier¹, Zoltan Cseresnyes^2,3, Juan Escribano Navarro⁴, Raluca A. Niesner², Anja E. Hauser^1,5

¹Immunodynamics,German Rheumatism Research Center, a Leibniz Institute ²Biophysical Analytics,German Rheumatism Research Center, a Leibniz Institute ³Max-Delbrück Center for Molecular Medicine ⁴Wimasis GmbH ⁵Immunodynamics and Intravital Imaging,Charité – University of Medicine

Summary

A strategy to quantitatively analyze histological data in the bone marrow is presented. Confocal microscopy of fluorescently labeled cells in tissue sections results in 2-dimensional images, which are automatically analyzed. Co-localization analyses of different cell types are compared to data from simulated images, giving quantitative information about cellular interactions.