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En Face Detection van stikstofoxide en superoxide in endotheellaag van Intact slagaders

Article DOI: 10.3791/53718-v • 08:58 min • February 25th, 2016
February 25th, 2016 •
1,2, 1,2, 2,3, 1,2, 1,2
1Cardiovascular and Aging Research, Department of Medicine, Division of Physiology, Faculty of Science, University of Fribourg, 2Kidney Control of Homeostasis, Swiss National Centre of Competence in Research, 3System Physiology, Department of Medicine, Division of Physiology, Faculty of Science, University of Fribourg

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In dit artikel beschrijven we een betrekkelijk eenvoudige manier aangepast met de fluorescentie kleurstof diaminofluorescein 2-diacetaat (DAF-2DA) en dihydroethidium (DHE) voor en face gelijktijdige detectie en visualisatie van intracellulaire stikstofoxide (NO) en superoxide anion (O 2 .- ) respectievelijk in vers geïsoleerde intacte aorta's van een obesitas muismodel.

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Molecular Biology bloedvaten confocale microscopie DHE DAF-2DA endotheel stikstofoxide superoxide Vasculaire Biologie
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