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Biochemistry

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JoVE Journal Biochemistry
Het meten van G-eiwit-gekoppelde Receptor Signaling via Radio-gelabeld GTP Binding
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Het meten van G-eiwit-gekoppelde Receptor Signaling via Radio-gelabeld GTP Binding

Article DOI: 10.3791/55561-v • 10:13 min • June 9th, 2017
June 9th, 2017 •
*1, *1, 1, 1,2, 1,3,4
1The Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, 2Department of Neurology and neurosurgery, Johns Hopkins University School of Medicine, 3Departments of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, 4Department of Psychiatry and Behavioral Sciences, Johns Hopkins University School of Medicine
* These authors contributed equally

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Guanosine trifosfaat (GTP) binding is een van de vroegste gebeurtenissen bij G-proteïne-koppelde receptor (GPCR) activatie. Dit protocol beschrijft hoe u specifieke GPCR-ligandinteracties farmacologisch kunt karakteriseren door de binding van het radioactief gemerkte GTP-analoog, [35S] guanosine-5'-O- (3-thio) trifosfaat ([35S] GTPγS) te monitoren in Reactie op een ligand van interesse.

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Biochemie Uitgave 124 Cellulaire fractionering membraanpreparatie GPCR agonist antagonist GTPγS binding farmacologie μ-opioïde receptor
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