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Moleculaire analyse van endotheel-mesenchymale overgang geïnduceerd door transformeren groeifactor-β signalering

Article DOI: 10.3791/57577-v • 07:49 min • August 3rd, 2018
August 3rd, 2018 •
1, 2,3, 4, 2
1David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, 2Department of Respiratory Medicine, Graduate School of Medicine, The University of Tokyo, 3Hastings Center for Pulmonary Research, Division of Pulmonary, Critical Care and Sleep Medicine, Department of Medicine, Keck School of Medicine, University of Southern California, 4Department of Molecular Pathology, Graduate School of Medicine, The University of Tokyo

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Een protocol voor in vitro inductie van endotheel-mesenchymale overgang (EndMT), die nuttig is voor het onderzoeken van cellulaire signaalroutes die betrokken zijn bij EndMT, wordt beschreven. In dit experimenteel model wordt EndMT veroorzaakt door behandeling met TGF-β in MS-1 endotheliale cellen.

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Biologie kwestie 138 endotheliale-mesenchymale EndMT endotheel overgangscel TGF-β α-SMA collageen microRNA
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