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Biology

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Visualisere samspillet mellem Qdot-mærket Protein og Site-specifically modificeret λ DNA på enkelt-molekyle niveau
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Visualisere samspillet mellem Qdot-mærket Protein og Site-specifically modificeret λ DNA på enkelt-molekyle niveau

Article DOI: 10.3791/57967-v • 08:56 min • July 17th, 2018
July 17th, 2018 •
*1,2, *1, 1,2, 1,2
1State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, 2Savaid Medical School, University of Chinese Academy of Sciences
* These authors contributed equally

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Vi præsenterer her, en protokol for at studere DNA-protein interaktioner af total interne reflection Fluorescens mikroskopi (TIRFM) ved hjælp af en site-specifically modificerede λ DNA substrat og en kvante-dot mærket protein.

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Biologi sag 137 enkelt molekyle imaging DNA-protein interaktion fluorescerende sonder Quantum dot total interne reflection Fluorescens mikroskopi λ DNA lokationsspecifikke ændring
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