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Visualizing the Interaction Between the Qdot-labeled Protein and Site-specifically Modified λ DNA at the Single Molecule Level

Article DOI: 10.3791/57967-v • 08:56 min • July 17th, 2018
July 17th, 2018 •
*1,2, *1, 1,2, 1,2
1State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, 2Savaid Medical School, University of Chinese Academy of Sciences
* These authors contributed equally

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Here, we present a protocol to study DNA-protein interactions by total internal reflection fluorescence microscopy (TIRFM) using a site-specifically modified λ DNA substrate and a Quantum-dot labeled protein.

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Qdot-labeled Protein Site-specific Modification DNA Single Molecule Level Single Molecule Imaging DNA Protein Interactions DNA Replication Gene Repair Chromosome Structure Modified Lambda DNA Flow Cell Assembly Coverslip Cleaning Ethanol Sonication Ultrapure Water Rinse Potassium Hydroxide Sonication Acetone Sonication Piranha Solution Incubation Methanol Rinse
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