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Journal / Biochemistry / Ekstracellulære Protein Microarray teknologi til…
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Biochemistry

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JoVE Journal Biochemistry
Ekstracellulære Protein Microarray teknologi til High Throughput påvisning af lav affinitet Receptor-Ligand interaktioner
A Modified Yeast-one Hybrid System for Heteromeric Protein Complex-DNA Interaction Studies
Journal (Biochemistry)
A Modified Yeast-one Hybrid System for Heteromeric Protein Complex-DNA Interaction Studies
Advancing EVtrap for High-Throughput Proteomics in Disease Biomarker Discovery (Video) | JoVE
Journal (Biochemistry)
Advancing EVtrap for High-Throughput Proteomics in Disease Biomarker Discovery (Video) | JoVE
 
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Ekstracellulære Protein Microarray teknologi til High Throughput påvisning af lav affinitet Receptor-Ligand interaktioner

Article DOI: 10.3791/58451-v • 06:01 min • January 7th, 2019
January 7th, 2019 •
1, 1, 2, 1
1Receptor Discovery group, Microchemistry, Proteomics and Lipidomics Department, Genentech, 2Portfolio Management and Operations, Genentech

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Vi præsenterer her, en protokol til skærmen ekstracellulære protein microarrays for identifikation af roman receptor-ligand interaktioner i høj overførselshastighed. Vi beskriver også en metode til at forbedre afsløring af forbigående protein-protein interaktioner ved hjælp af protein-microbead komplekser.

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Biokemi spørgsmålet 143 Protein microarray teknologi celleoverfladen receptor ekstracellulære protein protein-protein interaktioner bibliotek lav affinitet receptor opdagelse multimerization
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