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Effet des protéines fluorescentes sur des partenaires de Fusion utilisant les épreuves de toxicité Polyglutamine chez la levure

Article DOI: 10.3791/58748-v • 09:23 min • November 28th, 2018
November 28th, 2018 •
1, 2, 1, 1,2, 1
1Department of Anatomy and Cell Biology, University of Western Ontario, 2Department of Pathology and Laboratory Medicine, University of Western Ontario

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Cet article décrit les protocoles afin d’évaluer l’effet des protéines fluorescentes sur l’agrégation et la toxicité de l’expansion de polyglutamine mal repliées pour l’évaluation rapide d’une protéine fluorescente nouvellement indéterminée dans le contexte de reporters fluorescents.

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Biologie numéro 141 protéines fluorescentes polyglutamine toxicité essais de croissance de levure agrégation protéine fluorescente verte microscopie fluorescente
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