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Journal / Genetics / 酵母神经退行性蛋白病模型中组蛋白后转化修饰改变的表征…
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Genetics

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JoVE Journal Genetics
酵母神经退行性蛋白病模型中组蛋白后转化修饰改变的表征
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酵母神经退行性蛋白病模型中组蛋白后转化修饰改变的表征

Article DOI: 10.3791/59104-v • 08:33 min • March 24th, 2019
March 24th, 2019 •
1,2, 1,3, 1, 1, 1, 1, 1,4
1Chemistry Department, Brooklyn College, 2Ph.D. Program in Biochemistry, Graduate Center of the City University of New York, 3Ph.D. Program in Chemistry, Graduate Center of the City University of New York, 4Ph.D. Programs in Chemistry, Biochemistry, and Biology, Graduate Center of the City University of New York

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该协议概述了实验程序, 以描述全基因组的变化, 在与 ALS 和帕金森病相关的蛋白质过度表达的情况下, 组蛋白翻译后修饰 (PTM) 的水平发生。酿酒酵母模型。SDS-PAGE 分离后, 通过西方印迹, 通过修正特异性抗体检测单个组蛋白 PTM 水平。

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遗传学 第145期 神经退行性硬化 肌萎缩侧索硬化症 帕金森病 α-合核蛋白 融合在肉瘤 TAR dna 结合蛋白 43 组蛋白翻译后修饰 表观遗传学
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