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Inducerande och karaktäriserande vesikulär steatos i differentierade HepaRG-celler
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Inducerande och karaktäriserande vesikulär steatos i differentierade HepaRG-celler

Article DOI: 10.3791/59843-v • 09:15 min • July 18th, 2019
July 18th, 2019 •
1,2, 3, 3, 3, 2,4, 2,3,5,6, 1,3
1Department of Molecular Medicine, Sapienza University, 2Department of Internal Medicine - DMISM, Sapienza University, 3Center for Life Nano Science@Sapienza, Istituto Italiano di Tecnologia, 4Pasteur Institute Italy-Fondazione Cenci Bolognetti, 5INSERM U1052-Cancer Research Center of Lyon, 6Department of Hepatology, Croix Rousse Hospital, Hospices Civils de Lyon

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I denna studie, vi beskriver ett detaljerat protokoll för att inducera lever vesikulär steatos i differentierade HepaRG celler med fettsyra salt natriumoleat och använda metoder för detektion och kvantifiering av lipidackumulering, inklusive sammanhängande anti-Stokes Raman spridning (bilar) mikroskopi, cytofluorimetrisk analys, olja röd O färgning, och qPCR.

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Medicin heparg lever vesikulär steatos NAFLD lipid droppar bilar mikroskopi
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