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Journal / Developmental Biology / Hemogene herprogrammering van menselijke…
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Developmental Biology

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JoVE Journal Developmental Biology
Hemogene herprogrammering van menselijke fibroblasten door afgedwongen expressie van transcriptiefactoren
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Hemogene herprogrammering van menselijke fibroblasten door afgedwongen expressie van transcriptiefactoren

Article DOI: 10.3791/60112-v • 11:42 min • November 4th, 2019
November 4th, 2019 •
1,2,3, 3, 4, 5,6, 1,2,3
1Molecular Medicine and Gene Therapy, Lund Stem Cell Center, Lund University, 2Wallenberg Center for Molecular, Lund University, 3Center for Neuroscience and Cell Biology, University of Coimbra, 4Skolkovo Institute of Science and Technology, 5Department of Cell, Developmental and Regenerative Biology, Icahn School of Medicine at Mount Sinai, 6Black Family Stem Cell Institute, Icahn School of Medicine at Mount Sinai

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Dit protocol demonstreert de inductie van een hemogeen programma in menselijke dermale fibroblasten door afgedwongen expressie van de transcriptiefactoren GATA2, GFI1B en FOS om hematopoietische stam en voorlopercellen te genereren.

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Ontwikkelingsbiologie uitgave 153 hemogene herprogrammering fibroblast transcriptiefactoren Hematopoiesis hematopoietische stam en voorlopercellen endothelial-to-hematopoietische overgang
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