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JoVE Journal Cancer Research
Etablierung und Charakterisierung von kleinen Darm Neuroendokrinen Tumor Sphäriden
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Etablierung und Charakterisierung von kleinen Darm Neuroendokrinen Tumor Sphäriden

Article DOI: 10.3791/60303-v • 09:43 min • October 14th, 2019
October 14th, 2019 •
1, 1, 2, 3, 3, 1
1Department of Surgery, University of Iowa Carver College of Medicine, 2High Throughput Screening Facility, University of Iowa Carver College of Medicine, 3Department of Pathology, University of Iowa Carver College of Medicine

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Neuroendokrine Tumoren (NETs) stammen aus neuroendokrinen Zellen des Neuralkamms. Sie sind langsam wachsend und herausfordernd für die Kultur. Wir präsentieren eine alternative Strategie, um NETs aus dem kleinen Darm zu züchten, indem wir sie als Sphäroide kultivieren. Diese Sphäroide haben kleine Darm-NET-Marker und können für Drogentests verwendet werden.

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Krebsforschung Ausgabe 152 Neuroendokrine Kleindarmtumoren Sphäroide Synaptophysin Chromogranin A SSTR2 Immunfluoreszenz Immunhistochemie extrazelluläre Matrix Rapamycin
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