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1Center for Biological Systems Analysis,University of Freiburg, 2Faculty of Biology,University of Freiburg, 3Freiburg Institute for Advanced Studies (FRIAS),University of Freiburg, 4BIOSS Centre for Biological Signalling Studies,University of Freiburg, 5IMTEK Department of Microsystems Engineering,University of Freiburg, 6Cluster of Excellence livMatS at FIT – Freiburg Center for Interactive Materials and Bioinspired Technologies,University of Freiburg
Capitoli
- 00:05Introduction
- 00:45Protein Expression and Preparation
- 03:38Dye-modification of Elastin-like Proteins via SPAAC
- 04:44THF Swelling Protocol
- 06:29BuOH Extrusion Protocol
- 07:19Results: THF Swelling Method and the BuOH Extrusion Method for the Guided Self-assembly of Amphiphilic ELPs into Supramolecular Structures
- 09:22Conclusion
Organik ve sulu çözücülerin arayüzünde, özel ampifilik elastin benzeri proteinler veziküller, lifler ve çevresel parametrelertarafından tetiklenen koaksivatlar gibi karmaşık supramoleküler yapılarda bir araya getirilir. Açıklanan montaj protokolleri, çeşitli kargoların kapsüllemesini sağlayan, tunable özelliklere sahip Protein Membran Tabanlı Bölmeler (PMBCs) verir.
