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In situ Hybridization for Sipunculus nudus Coelomic Fluid
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Biologia
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JoVE Journal Biologia
In situ Hybridization for Sipunculus nudus Coelomic Fluid

In situ Hybridization for Sipunculus nudus Coelomic Fluid

DOI:
10.3791/61022-v

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07:35 min

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May 01, 2020

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, ,
1Key Laboratory of Xiamen Marine and Gene Drugs, School of Biomedical Sciences,Huaqiao University

Capitoli

  • 00:00Introduction
  • 00:44Collection of Coelomic Fluid
  • 01:33In situ Hybridization: Day 1
  • 03:20In situ Hybridization: Day 2
  • 05:10In situ Hybridization: Day 3
  • 06:17Risultati
  • 07:15Conclusion

Summary

Traduzione automatica

Traduzione automatica

This protocol describes an effective in situ hybridization approach to detect the mRNA expression levels and spatial patterns of target genes in Sipunculus nudus coelomic fluid.

Tags

In Situ HybridizationSipunculus NudusCoelomic FluidMRNA ExpressionLncRNA ExpressionGene LocalizationFishery ResourceRNA ProbesProteinase KPFA FixationWash BuffersAntibody StainingAlkaline PhosphataseBCIP-NBT Staining

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