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Journal / Neuroscience / Combinatie van multiplex fluorescentie in situ…
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Neuroscience

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JoVE Journal Neuroscience
Combinatie van multiplex fluorescentie in situ hybridisatie met fluorescerende immunohistochemie op vers ingevroren of vaste hersensecties van muizen
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Combinatie van multiplex fluorescentie in situ hybridisatie met fluorescerende immunohistochemie op vers ingevroren of vaste hersensecties van muizen

Article DOI: 10.3791/61709-v • 07:36 min • June 25th, 2021
June 25th, 2021 •
1, 1, 1
1Department of Pharmacology, School of Medical Sciences, University of New South Wales

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Dit protocol beschrijft een methode voor het combineren van fluorescentie in situ hybridisatie (FISH) en fluorescentie immunohistochemie (IHC) in zowel vers ingevroren als gefixeerde hersensecties van muizen, met als doel het bereiken van multilabel FISH en fluorescentie IHC-signaal. IHC richtte zich op cytoplasmatische en membraangebonden eiwitten.

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Multiplex fluorescentie in situ hybridisatie FISH fluorescerende immunohistochemie vers ingevroren hersensecties van muizen vaste hersensecties van muizen RNA-transcripten neurochemische fenotypering antilichamen eiwitetikettering RNAscope FISH fluorescentie-immunokleuring galaninereceptor 1 glycinetransporter 2 hersenstamkernen
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