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Genoomtechniek van primaire menselijke B-cellen met CRISPR/Cas9

Article DOI: 10.3791/61855-v • 08:20 min • November 3rd, 2020
November 3rd, 2020 •
1,2,3, 1,2,3, 1,2,3, 1,2,3, 1,2,3, 1,2,3
1Department of Pediatrics, University of Minnesota, 2Center for Genomic Engineering, University of Minnesota, 3Masonic Cancer Center, University of Minnesota

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Hier bieden we een gedetailleerd, stapsgewijs protocol voor CRISPR/ Cas9-gebaseerde genoomengineering van primaire menselijke B-cellen voor gen knock-out (KO) en knock-in (KI) om biologische functies van genen in B-cellen en de ontwikkeling van B-celtherapieën te bestuderen.

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Biologie CRISPR/Cas9 genoomtechniek recombinante AAV genbewerking primaire menselijke B-cellen
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