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新型人类组织特征视网膜培养技术的特征

JoVE Journal
Medicina

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JoVE Journal Medicina

Characterization of a Novel Human Organotypic Retinal Culture Technique

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新型人类组织特征视网膜培养技术的特征

DOI:

10.3791/62046-v

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05:51 min

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June 09, 2021

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Charisse Y. J. Kuo*¹, Henry H. Louie, Ilva D. Rupenthal, Odunayo O. Mugisho*¹

¹Buchanan Ocular Therapeutics Unit, Department of Ophthalmology and the New Zealand National Eye Centre,University of Auckland

Capitoli

00:05Introduction
00:53Media Preparation and Extraction of the Sandwich Retinal Explants from the Eyecup
03:02Results: Observation of Cellular Integrity of Retinal Explants Using H&E, IHC and Analysis of Cytokine Release Using Magnetic Assay
04:53Conclusion

Summary

Traduzione automatica

这项研究旨在开发一种新的人体组织性视网膜培养（HORC）模型，防止在外植过程中损害视网膜的完整性。这是通过培养视网膜与过度的四肢和底层视网膜色素上皮-胆碱（RPE-胆碱）和硬膜。

Tags

Organotypic Retinal Culture Retinal Explant Retinal Integrity Retinal Diseases Drug Testing Dulbecco’s Modified Eagle Medium Antibiotics Antimycotics Dissection Sample Collection Sclera Optic Nerve Head

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