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Établissement et manipulation génétique d’organoïdes d’hépatocytes murins
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Établissement et manipulation génétique d’organoïdes d’hépatocytes murins

Article DOI: 10.3791/62438-v • 14:54 min • February 12th, 2022
February 12th, 2022 •
*1,2, *1,3, 1,2, 1,2
1Key Laboratory of Experimental Teratology, Ministry of Education, Institute of Molecular Medicine and Genetics, School of Basic Medical Sciences, Stem Cell and Regenerative Medicine Research Center, Cheeloo College of Medicine, Shandong University, 2The Research Center of Stem Cell and Regenerative Medicine, Shandong University Cheeloo Medical College, School of Basic Medical Sciences, Shandong University
* These authors contributed equally

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Un système de culture organoïde 3D ex vitro à long terme a été établi à partir d’hépatocytes de souris. Ces organoïdes peuvent être passés et génétiquement manipulés par l’infection à lentivirus de la construction shRNA/ectopique, la transfection de siRNA et l’ingénierie CRISPR-Cas9.

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Biologie numéro 180
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