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Estabelecimento e Manipulação Genética de Organoides Hepatocitos De Murine
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Estabelecimento e Manipulação Genética de Organoides Hepatocitos De Murine

Article DOI: 10.3791/62438-v • 14:54 min • February 12th, 2022
February 12th, 2022 •
*1,2, *1,3, 1,2, 1,2
1Key Laboratory of Experimental Teratology, Ministry of Education, Institute of Molecular Medicine and Genetics, School of Basic Medical Sciences, Stem Cell and Regenerative Medicine Research Center, Cheeloo College of Medicine, Shandong University, 2The Research Center of Stem Cell and Regenerative Medicine, Shandong University Cheeloo Medical College, School of Basic Medical Sciences, Shandong University
* These authors contributed equally

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Um sistema de cultura organoide 3D ex vitro de longo prazo foi estabelecido a partir de hepatócitos de camundongos. Esses organoides podem ser passagemdos e geneticamente manipulados por infecção por lentivírus de construção shRNA/ectópica, transfecção de siRNA e engenharia CRISPR-Cas9.

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Biologia Edição 180
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