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JoVE Journal Cancer Research
Tumörtransplantation för att bedöma dynamiken hos tumörinfiltrerande CD8 + T-celler hos möss
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Tumörtransplantation för att bedöma dynamiken hos tumörinfiltrerande CD8 + T-celler hos möss

Article DOI: 10.3791/62442-v • 07:36 min • June 12th, 2021
June 12th, 2021 •
1, 1, 2, 1, 2, 1, 3, 1, 1, 1, 1, 1, 1, 1, 1, 4, 1, 5, 1
1Institute of Immunology, Third Military Medical University, 2Guanghua School of Stomatology, Guangdong Provincial Key Laboratory of Stomatology, Stomatological Hospital, Sun Yat-Sen University, 3Shigatse Branch, Xinqiao Hospital, Third Military Medical University, 4Department of Emergency Medicine, Southwest Hospital, Third Military Medical University, 5Cancer Center, The General Hospital of Western Theater Command

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Här presenterar vi ett tumörtransplantationsprotokoll för karakterisering av tumör-inneboende och periferi-härledda tumörinfiltrerade lymfocyter i en mustumörmodell. Specifik spårning av tillströmningen av mottagarhärledda immunceller med flödescytometri avslöjar dynamiken i de fenotypiska och funktionella förändringarna hos dessa celler under antitumörimmunsvar.

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Cancerforskning utgåva 172
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