È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
1Centre for Reproductive Medicine, Department of Obstetrics and Gynecology,Peking University Third Hospital, 2National Clinical Research Center for Obstetrics and Gynecology(Peking University Third Hospital), 3Ministry of Education,Key Laboratory of Assisted Reproduction (Peking University), 4Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology, 5Department of Clinical Research,Yikon Genomics Co. Ltd.
Capitoli
- 00:04Introduction
- 01:24Preparation and Denudation of Granulosa Cells, Oocyte Evaluation and Embryo Washing
- 03:15Culture Medium Collection Options 1 and 2, Sample Collection
- 05:12Culture Medium Lysis
- 06:22Pre‐Library Preparation
- 07:24Library Preparation
- 08:14Library Purification and Quantification, Sequencing and Data Analysis
- 09:43Results: Embryo Screening and Selection Using NICS from a Patient
- 11:08Conclusion
Den aktuella studien rapporterar ett protokoll för kromosomscreening av mänskliga embryon som använder använt odlingsmedium, vilket undviker embryobiopsi och möjliggör kromosomploiditetsidentifiering med hjälp av NGS. Den här artikeln presenterar den detaljerade proceduren, inklusive beredning av odlingsmedium, helgenomamplifiering (WGA), förberedelse av nästa generations sekvenseringsbibliotek (NGS) och dataanalys.
