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Isolamento delle cellule endoteliali dal lume delle arterie carotidi di topo per esperimenti multi-omici a singola cellula

Article DOI: 10.3791/63128-v • 09:12 min • October 4th, 2021
October 4th, 2021 •
*1, *1, 1, 1, 1, 1,2
1Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, 2Division of Cardiology, Georgia Institute of Technology and Emory University
* These authors contributed equally

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Presentiamo un metodo per isolare cellule e nuclei endoteliali dal lume delle arterie carotidi di topo esposte a condizioni di flusso stabili o disturbate per eseguire esperimenti omici a singola cellula.

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Biologia Numero 176 Cellule arteriose Cellule endoteliali RNAseq unicellulare ATACseq unicellulare Aterosclerosi
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