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Visualisation simultanée de la dynamique des microtubules réticulés et simples in vitro par microscopie TIRF

Article DOI: 10.3791/63377-v • 07:20 min • February 18th, 2022
February 18th, 2022 •
*1,2, *1, 1,2
1Molecular Biology, Massachusetts General Hospital, 2Department of Genetics, Harvard Medical School
* These authors contributed equally

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Ici, un essai de reconstitution in vitro basé sur la microscopie TIRF est présenté pour quantifier et comparer simultanément la dynamique de deux populations de microtubules. Une méthode est décrite pour visualiser simultanément l’activité collective de plusieurs protéines associées aux microtubules sur des faisceaux de microtubules réticulés et des microtubules simples.

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Biochimie numéro 180
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