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Biochemistry

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使用200 KV透射电子显微镜常规收集高分辨率冷冻电镜数据集
 
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使用200 KV透射电子显微镜常规收集高分辨率冷冻电镜数据集

Article DOI: 10.3791/63519-v 09:49 min March 16th, 2022
March 16th, 2022

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大分子的高分辨率冷冻电镜图谱也可以通过使用200 kV透射显微镜来实现。该协议显示了设置精确光学对准、数据采集方案和成像区域选择的最佳实践,这些对于使用200 kV TEM成功收集高分辨率数据集至关重要。

Tags

生物化学,第181期,冷冻电镜,高分辨率,结构,对齐,数据收集,数据管理,冰川,EPU,能量过滤器
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