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Genom-dækkende profilering af transkriptionsfaktor-DNA-bindende interaktioner i Candida albicans: En omfattende CUT &RUN-metode og dataanalysearbejdsgang

Article DOI: 10.3791/63655-v • 07:48 min • April 1st, 2022
April 1st, 2022 •
*1,2, *1,2, *1,2, 1,2,4, 3, 1,4, 1,4
1Department of Molecular and Cell Biology, University of California, Merced, 2Quantitative and Systems Biology Graduate Program, University of California, Merced, 3Department of Applied Mathematics, University of California, Merced, 4Health Sciences Research Institute, University of California, Merced
* These authors contributed equally

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Denne protokol beskriver en eksperimentel metode og dataanalysearbejdsgang for spaltning under mål og frigivelse ved hjælp af nuklease (CUT & RUN) i det humane svampepatogen Candida albicans.

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Biologi Udgave 182 Candida albicans CUT&RUN transkriptionsfaktorer genom-dækkende bindingsmetoder transkriptionel regulering
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