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Stabilire organoidi polmonari umani e differenziazione prossimale per generare organoidi maturi delle vie aeree

Article DOI: 10.3791/63684-v • 10:12 min • March 23rd, 2022
March 23rd, 2022 •
1,2, 1,2, 1,2, 1,2, 1,2, 1,2, 1,2, 1,2,3
1Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, 2Centre for Virology, Vaccinology and Therapeutics, Hong Kong Science and Technology Park, 3State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong

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Il protocollo presenta un metodo per derivare organoidi polmonari umani da tessuti polmonari primari, espandere gli organoidi polmonari e indurre la differenziazione prossimale per generare organoidi delle vie aeree 3D e 2D che fenocopio fedelmente l'epitelio delle vie aeree umane.

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Biologia Numero 181
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