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JoVE Journal Neuroscience
Hippocampale nevronkulturer for å oppdage og studere nye patogene antistoffer involvert i autoimmun encefalitt
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Hippocampale nevronkulturer for å oppdage og studere nye patogene antistoffer involvert i autoimmun encefalitt

Article DOI: 10.3791/63829-v • 08:20 min • June 2nd, 2022
June 2nd, 2022 •
1, 2, 1, 1,2
1ICFO-Institut de Ciències Fotòniques, The Barcelona Institute of Science and Technology, 2Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Hospital Clínic, Universitat de Barcelona

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Autoimmun encefalitt er en ny kategori av antistoffmedierte sykdommer i sentralnervesystemet. Hippocampale nevroner kan brukes til å oppdage og karakterisere disse antistoffene. Denne artikkelen gir en protokoll for primær cellekultur og immunostaining for å bestemme autoantistoffer i serum og cerebrospinalvæske hos pasienter.

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