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Journal / Developmental Biology / FACS-aislamiento y cultivo de progenitores…
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Developmental Biology

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JoVE Journal Developmental Biology
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FACS-aislamiento y cultivo de progenitores fibroadipogénicos y células madre musculares a partir de músculo esquelético de ratón imperturbable y lesionado

Article DOI: 10.3791/63983-v • 11:25 min • June 8th, 2022
June 8th, 2022 •
1, 2, 1
1Laboratory of Muscle Stem Cells and Gene Regulation, National Institute of Arthritis, Musculoskeletal, and Skin Diseases (NIAMS), National Institutes of Health (NIH), 2Flow Cytometry Section, National Institute of Arthritis, Musculoskeletal, and Skin Diseases (NIAMS), National Institutes of Health (NIH)

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La identificación precisa de las células progenitoras fibroadipogénicas (FAP) y las células madre musculares (MuSC) es fundamental para estudiar su función biológica en condiciones fisiológicas y patológicas. Este protocolo proporciona pautas para el aislamiento, purificación y cultivo de FAP y MuSC de músculos de ratones adultos.

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Developmental Biology Número 184 progenitores fibroadipogénicos musculares células madre musculares (MuSCs) células madre mesenquimales células estromales mesenquimales FACS regeneración muscular
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