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Cancer Research

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工业化、人工智能引导的激光显微切割,用于肿瘤微环境的微尺度蛋白质组学分析
 
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工业化、人工智能引导的激光显微切割,用于肿瘤微环境的微尺度蛋白质组学分析

Article DOI: 10.3791/64171-v 13:01 min June 3rd, 2022
June 3rd, 2022

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该协议描述了一种高通量工作流程,用于人工智能驱动的从染色的薄组织切片图像中分割病理确认感兴趣的区域,以使用激光显微切割富集组织学分辨细胞群。该策略包括一种新颖的算法,能够将表示感兴趣细胞群的分界线直接转移到激光显微镜上。

Tags

癌症研究,第184期,组织病理学图像分析,激光显微切割,新鲜冷冻组织,福尔马林固定石蜡包埋组织,压力循环技术,串联质量标签,定量蛋白质组学
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