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Journal / Developmental Biology / AMEBaS：偏振单细胞比例荧光延时的自动中线提取和背景减法…
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Developmental Biology
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JoVE Journal Developmental Biology
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AMEBaS：偏振单细胞比例荧光延时的自动中线提取和背景减法

Article DOI: 10.3791/64857-v • 06:03 min • June 23rd, 2023
June 23rd, 2023 •
1, 1,2, 3, 4, 5, 6, 1, 1
1Institute of Mathematics and Statistics, University of São Paulo, 2Center for Mathematics, Computing and Cognition, Federal University of ABC, 3Department of Botany, Institute of Biosciences, University of São Paulo, 4Cell Biology and Molecular Genetics Department, University of Maryland, 5Department of Biosciences, University of Milan, 6Department of Physics, Politecnico di Milano

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目前用于分析极化单细胞细胞内动力学的方法通常是手动的，缺乏标准化。本文介绍了一种新颖的图像分析管道，用于在用户友好的在线界面中自动提取单偏振细胞的中线，并量化时间流逝的时空行为。

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发育生物学，第 196 期，背景减法，比率荧光延时摄影，偏振单细胞，细胞极性，宏观现象，空间集中分子，特化域，亚细胞水平，不对称形态结构，细胞分裂，生长，迁移，细胞极性破坏，组织相关疾病，癌症，胃发育不良，时空动力学，荧光报告基因，手动步骤，中线追踪，耗时，偏差，比率分析、报告分子分布不均匀、背景减法技术、计算管道、自动化和量化时空行为、单细胞、花粉管/根毛生长、胞质离子动力学
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