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Mikrovaskulær og makrovaskulær endotelcelleisolering og oprensning fra lungeafledte prøver
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Mikrovaskulær og makrovaskulær endotelcelleisolering og oprensning fra lungeafledte prøver

Article DOI: 10.3791/64885-v • 05:26 min • February 3rd, 2023
February 3rd, 2023 •
1,2, 2, 2,3, 2,3, 1, 4, 4,5, 1,2, 2,3, 1, 6,7, 1, 1,2
1Department of Medical, Oral and Biotechnological Sciences, “G. d’Annunzio” University of Chieti-Pescara, 2Center on Advanced Studies and Technology (CAST), “G. d’Annunzio” University of Chieti-Pescara, 3Department of Medicine and Aging Sciences, University “G. d’Annunzio” of Chieti-Pescara, 4U.O. di Chirurgia Toracica e dei Trapianti di Polmone, Fondazione IRCCS Ca’ Granda - Ospedale Maggiore Policlinico di Milano, 5Dipartimento di Fisiopatologia Medico-Chirurgica e dei Trapianti, Università degli Studi di Milano, 6Department of Pharmaceutical Sciences, Università degli Studi di Milano, 7Unit of Cell and Molecular Biology in Cardiovascular Diseases, Centro Cardiologico Monzino IRCCS

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Selvom det er udfordrende, er isolering af lungeendotelceller afgørende for undersøgelser af lungebetændelse. Denne protokol beskriver en procedure til højtydende isolering af makrovaskulære og mikrovaskulære endotelceller med høj renhed.

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Denne måned i JoVE udgave 192 Endotelceller explant endotel lunger celleisolering betændelse
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