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Biochemistry

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JoVE Journal Biochemistry
Semi-geautomatiseerde fenotypische analyse van functionele 3D-sferoïde celculturen
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Semi-geautomatiseerde fenotypische analyse van functionele 3D-sferoïde celculturen

Article DOI: 10.3791/65086-v • 06:48 min • August 18th, 2023
August 18th, 2023 •
1, 1, 2,3, 2, 3, 1
1Department of Biochemistry, Albert Einstein College of Medicine, 2Department of Biochemistry and Molecular Biology, University of Southern Denmark, 3CelVivo ApS

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We presenteren een protocol voor het kweken van hoogreproduceerbare sferoïden en hun fenotypische karakterisering met behulp van beeldopname en proteomics.

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Biochemie Nummer 198 Clinostat Incubator 3D-celculturen Kankerhepatocyten Niet-kankerhepatocyten Levercellen Incubators voor 3D-cellen Celgroeibewaking Momentopnamen van celgroei Sferoïde tellen Sferoïde meting THLE-3-cellijn HepG2/C3A-cellijn Proteomics-experimenten Verstoring van celsignalering Metabolisme van de ademhalingsketen Metaalontgifting Fenotypische karakterisering Image Capture Proteomics-pijplijn
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