JoVE Journal

Biology

JoVE Biology includes techniques and experimental approaches in the fields of cellular, molecular, and organismal biology.

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Author Spotlight: Optimizing Cryo-EM Analysis with CryoSieve for Enhanced Particle Selection Efficiency

Mengjia Cai*¹, Jianying Zhu*², Qi Zhang*^3,4,5,6, Yu Xu⁷, Zuoqiang Shi^2,8, Chenglong Bao^2,8,9, Mingxu Hu^1,5,6

¹Institute of Bio-Architecture and Bio-Interactions (IBABI), Shenzhen Medical Academy of Research and Translation, ²Yau Mathematical Sciences Center, ³Key Laboratory of Protein Science (Tsinghua University), Ministry of Education, ⁴School of Life Science, Tsinghua University, ⁵Beijing Advanced Innovation Center for Structural Biology, ⁶Beijing Frontier Research Center for Biological Structure, ⁷Department of Mathematical Sciences, Tsinghua University, ⁸Yanqi Lake Beijing Institute of Mathematical Sciences and Applications, ⁹State Key Laboratory of Membrane Biology, School of Life Science

JoVE 66617

Author Spotlight: Advancing Intestinal Bacteria Cultivation for Poultry

Harshita Naithani^1,2, Bibiana Rios-Galicia^1,2, Amélia Camarinha Silva^1,2, Jana Seifert^1,2

¹Institute of Animal Science, University of Hohenheim, ²HoLMiR - Hohenheim Center for Livestock Microbiome Research, University of Hohenheim

JoVE 66570

DNA Virus Detection System Based on RPA-CRISPR/Cas12a-SPM and Deep Learning

Changyue Liu*^1,2, Zhengyang Lei*^1,2, Lijin Lian², Likun Zhang^1,2, Zhicheng Du^1,2, Peiwu Qin^1,2

¹Center of Precision Medicine and Healthcare, Tsinghua-Berkeley Shenzhen Institute, ²Institute of Biopharmaceutics and Health Engineering, Tsinghua Shenzhen International Graduate School

JoVE 64833

Methods Collection

Author Spotlight: THP-1 Macrophage Response to LPS/ATP - Unveiling Pyroptosis, Apoptosis and Necroptosis Spectrum

Huan Wang¹, Yuejia Lan¹, Cuiping Chen¹, Lu Yang², Jiayi Sun², Yong Zeng¹, Jiasi Wu³

¹State Key Laboratory of Southwestern Chinese Medicine Resources, Chengdu University of Traditional Chinese Medicine, ²Innovative Institute of Chinese Medicine and Pharmacy, Chengdu University of Traditional Chinese Medicine, ³Acupuncture and Tuina School, Chengdu University of Traditional Chinese Medicine

JoVE 66831

Author Spotlight: Deciphering the Cellular Mysteries of Intermuscular Adipose Tissue in Humans

Line O. Elingaard-Larsen^1,2, Katie L. Whytock¹, Adeline Divoux¹, Meghan Hopf¹, Erin E. Kershaw³, Jamie N. Justice⁴, Bret H. Goodpaster¹, Nancy E. Lane⁵, Lauren M. Sparks¹

¹Translational Research Institute, AdventHealth, ²Steno Diabetes Center Copenhagen, ³Division of Endocrinology and Metabolism, University of Pittsburgh, ⁴Gerontology and Geriatric Medicine, Wake Forest University School of Medicine, ⁵Department of Internal Medicine - Rheumatology, Allergy, and Clinical Immunology, University of California Davis Health

JoVE 66784

Isolation and Culture of Primary Human Mammary Epithelial Cells

Linfeng Qian*¹, Jiaxi Yan*¹, Shiqi Chen¹, Maryam Mohanmmed Abbas Karekad², Yue Wu^3,4, Xunwei Wu⁵, Xiaohong Xu^3,4, Xiaoqun Zhang^6,7

¹The First Clinical Medical College of Zhejiang Chinese Medical University, ²The International Education College of Zhejiang Chinese Medical University, ³Department of Breast Surgery, The First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Traditional Chinese Medicine), ⁴Bozhou District Hospital of Traditional Chinese Medicine, ⁵Engineering Laboratory for Biomaterials and Tissue Regeneration, Ningbo Stomatology Hospital, Ningbo, China and Savaid Stomatology School, Hangzhou Medical College, ⁶Department of Surgery, Kaihua District, The First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Traditional Chinese Medicine), ⁷Kaihua County Hospital of Traditional Chinese Medicine

JoVE 66638

Author Spotlight: Modeling Retinal Pathologies with Enhanced RPE Cell-Based Disease Models

Chase A. Paterson¹, Dillon Weatherston¹, Teren Teeples¹, Elizabeth Vargis¹

¹Department of Biological Engineering, Utah State University

JoVE 66079

Author Spotlight: Use of Fish Scales for Bone Remodeling Research – Advancements in Ex Vivo Imaging and Dissecting Cell Interactions

Qiao Tong*¹, Renata Raele*¹, Dylan Bergen¹, Chrissy Hammond¹

¹School of Physiology, Pharmacology and Neuroscience, Biomedical Science Building, University of Bristol

JoVE 65396

Author Spotlight: Exploring Cytoskeletal Dynamics to Unveil Novel Antibiotics Through Innovative Cell-Based Assays

Sakshi Mahesh Poddar^1,2, Srijita Roy^1,2, Ajay Kumar Sharma^1,2, Ramanujam Srinivasan^1,2

¹School of Biological Sciences, National Institute of Science Education and Research, ²Homi Bhabha National Institutes

JoVE 66657

Author Spotlight: Establishing an Accurate Microhardness Testing Protocol for Craniofacial Tissues

Motoki Okamoto*¹, Shohei Yamashita*¹, Takumi Memida¹, Melanie Mendonca¹, Susanne Brueckner¹, Shin Nakamura¹, Satoru Shindo¹, Ria Achong-Bowe¹, Jeffrey Thompson¹, Xiaozhe Han¹, Toshihisa Kawai¹, Maiko Suzuki¹

¹Department of Oral Science and Translational Research, College of Dental Medicine, Nova Southeastern University

JoVE 66583

Author Spotlight: Optimized Method for Isolating Rat Neutrophils and NETs from Bone Marrow

Xiangfeng Gong*¹, Yutao Sun*¹, Xiaoxin Zhang^2,3, Zhenghua Xiao^1,4, Li He^1,4, Chaoyi Qin^1,4

¹Department of Cardiovascular Surgery, West China Hospital, Sichuan University, ²Department of Integrated Traditional Chinese and Western Medicine, Sichuan Provincial Pancreatitis Centre, West China Hospital, Sichuan University, ³West China-Liverpool Biomedical Research Centre, West China Hospital, Sichuan University, ⁴Cardiovascular Surgery Research Laboratory, West China Hospital, Sichuan University

JoVE 65506

Growing a Cystic Fibrosis-Relevant Polymicrobial Biofilm to Probe Community Phenotypes

Sarah Poirier¹, Fabrice Jean-Pierre¹

¹Département de Biologie, Université de Sherbrooke

JoVE 66785

Author Spotlight: Exploring Bradysia coprophila's Unique Biology: A Guide to Laboratory Maintenance

Susan A. Gerbi¹

¹Department of Molecular and Cell Biology and Biochemistry, Division of Biology and Medicine, Brown University

JoVE 66751

Author Spotlight: A Novel 3D-Printed Titanium Implant for Minimally Invasive Treatment of Hip Dysplasia in Young Dogs

Irin Kwananocha^1,2, Femke Verseijden¹, Seyed A Kamali¹, Joëll Magré³, Koen Willemsen³, Jacobine CM Schouten⁴, Daniela Salvatori⁴, Marianna A Tryfonidou¹, Björn P Meij¹

¹Department of Clinical Sciences, Faculty of Veterinary Medicine, Utrecht University, ²Research and Academic Service, Faculty of Veterinary Medicine, Kasetsart University, ³Department of Orthopedics, University Medical Center Utrecht, ⁴Department of Clinical Sciences, Anatomy and Physiology, Faculty of Veterinary Medicine, Utrecht University

JoVE 66005

Author Spotlight: Exploring Regeneration in Axolotls Through Insights in Cellular and Molecular Mechanisms, Bone Healing, and Implications for Human Therapies

Anastasia Polikarpova¹, Christian Bucher², Agnes Ellinghaus^2,3, Helena Okulski¹, Georg Duda², Katharina Schmidt-Bleek^2,3, Elly Margaret Tanaka¹

¹Research Institute of Molecular Pathology, ²Julius Wolff Institute for Biomechanics and Musculoskeletal Regeneration, Berlin Institute of Health at Charité - Universitätsmedizin Berlin, ³Berlin Institute of Health Centre for Regenerative Therapies, Berlin Institute of Health at Charité - Universitätsmedizin Berlin

JoVE 66648

Advancements in Stem Cell Regenerative Therapy Through Photobiomodulation

Brendon Roets¹, Heidi Abrahamse¹, Anine Crous¹

¹Laser Research Centre, Faculty of Health Science, University of Johannesburg

JoVE 66616

Photodynamic Therapy as a Novel Approach to Induce Petite Colonies in Drug-Resistant Candida for Antifungal Research

Cai-Ying Yang*^1,2, Jia-Horung Hung*³, Chi-Jung Wu^4,5, Zhao-Xiang Wang^1,2, Shih-Han Wang⁶, Hao-Chun Liaw², I-Huang Lin³, Chun-Keung Yu^1,6, Tak-Wah Wong^2,7,8

¹Department of Microbiology and Immunology, College of Medicine, National Cheng Kung University, ²Department of Dermatology, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, ³Department of Ophthalmology, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, ⁴National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, ⁵Department of Internal Medicine, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, ⁶Insititute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, ⁷Department of Biochemistry and Molecular Biology, College of Medicine, National Cheng Kung University, ⁸Center of Applied Nanomedicine, National Cheng Kung University