JoVE Journal

Medicine

JoVE Medicine includes methods and experimental approaches for biomedical research and clinical medicine, including case studies, clinical procedures, surgeries, clinical trials, and animal models of disease.

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Setup and Execution of the Rapid Cycle Deliberate Practice Death Notification Curriculum

Patrick G. Hughes¹, Kate E. Hughes², Mary J. Hughes^3,4, Lindsay Weaver⁴, Lauren E. Falvo⁴, Anna M. Bona⁴, Dylan Cooper⁴, Cherri Hobgood⁴, Rami A. Ahmed⁴

¹Florida Atlantic University Schmidt College of Medicine, ²Department of Emergency Medicine, University of Arizona College of Medicine, ³Department of Osteopathic Medical Specialties, College of Osteopathic Medicine, Michigan State University, ⁴Indiana University School of Medicine

JoVE 61646

3D Planning and Printing of Patient Specific Implants for Reconstruction of Bony Defects

Tal Capucha*¹, Dekel Shilo*^1,2, Ori Blanc¹, Shahar Turgeman¹, Omri Emodi^1,2, Adi Rachmiel^1,2

¹Department of Oral and Maxillofacial Surgery, Rambam Medical Care Center, ²Ruth & Bruce Rappaport Faculty of Medicine, Technion-Israel Institute of Technology

JoVE 60929

Applying a Three-dimensional Uniaxial Mechanical Stimulation Bioreactor System to Induce Tenogenic Differentiation of Tendon-Derived Stem Cells

Ziming Chen*¹, Peilin Chen*¹, Rui Ruan*¹, Lianzhi Chen¹, Jun Yuan¹, David Wood¹, Tao Wang¹, Ming Hao Zheng¹

¹Centre of Orthopaedic Translational Research, Medical School, University of Western Australia

JoVE 61278

Dynamic Measurement and Imaging of Capillaries, Arterioles, and Pericytes in Mouse Heart

Guiling Zhao¹, Humberto C. Joca¹, W. Jonathan Lederer¹

¹Laboratory of Molecular Cardiology, Center for Biomedical Engineering and Technology and Department of Physiology, University of Maryland School of Medicine

JoVE 61566

A Novel Right Ventricular Volume and Pressure Loaded Piglet Heart Model for the Study of Tricuspid Valve Function.

Lily Q. Lin¹, Sanaz Hatami², James Yashu Coe¹, Timothy M. Colen¹, Consolato Sergi³, Richard Thompson⁴, Elena S. Di Martino⁵, Walter Herzog⁶, Ziad Abu Sara⁶, Darren H. Freed², Nee Scze Khoo¹

¹Division of Pediatric Cardiology, Department of Pediatrics, University of Alberta, ²Department of Surgery, University of Alberta, ³Department of Laboratory Medicine and Pathology, University of Alberta, ⁴Department of Biomedical Engineering, University of Alberta, ⁵Department of Civil Engineering, University of Calgary, ⁶Faculty of Kinesiology, University of Calgary

JoVE 61251

A Mouse Model of Incompletely Resected Soft Tissue Sarcoma for Testing (Neo)adjuvant Therapies

Francois X. Rwandamuriye^1,2, Breana J. Weston^2,3, Terrance G. Johns², W. Joost Lesterhuis^1,2, Rachael M. Zemek²

¹School of Biomedical Sciences, University of Western Australia, ²Telethon Kids Institute, University of Western Australia, ³College of Science, Health, Engineering and Education, Murdoch University

JoVE 60882

A Saline/Bipolar Radiofrequency Energy Device As an Adjunct for Hemostasis in Solid Organ Injury/Trauma

Thomas W. Clements¹, Chad G. Ball¹

¹Department of Surgery, University of Calgary

JoVE 57333

Intratracheal Administration of Dry Powder Formulation in Mice

Yingshan Qiu¹, Qiuying Liao¹, Michael Y.T. Chow^1,2, Jenny K.W. Lam¹

¹Department of Pharmacology and Pharmacy, LKS Faculty of Medicine, The University of Hong Kong, ²Advanced Drug Delivery Group, Sydney Pharmacy School, Faculty of Medicine and Health, The University of Sydney

JoVE 61469

Endoscopy Guided Photoablation of Endometrial Cysts using a 980 nm Laser with a Contact Fiber in Mares

Jérôme Ponthier¹, Geoffroy de la Rebière², Alexandra Salciccia², Stéfan Deleuze¹

¹Equine Reproduction, Veterinary Teaching Hospital, Liège University, ²Equine Surgery, Veterinary Teaching Hospital, Liège University

JoVE 61569

Methods Collection

Use of an Integrated Low-Flow Anesthetic Vaporizer, Ventilator, and Physiological Monitoring System for Rodents

Krista Bigiarelli¹, Luke E. Schepers², Arvin H. Soepriatna², Dave FitzMiller³, Craig J. Goergen²

¹Pre-Clinical Research and Development, Kent Scientific Corporation, ²Weldon School of Biomedical Engineering, Purdue University, ³Marketing Research and Development, Kent Scientific Corporation

JoVE 61311

A Rat Carotid Artery Pressure-Controlled Segmental Balloon Injury with Periadventitial Therapeutic Application

Nicholas E. Buglak^1,2,3,5, Edward S. M. Bahnson^1,2,3,4,5

¹Department of Surgery, Division of Vascular Surgery, University of North Carolina at Chapel Hill, ²Center for Nanotechnology in Drug Delivery, University of North Carolina at Chapel Hill, ³Curriculum in Toxicology & Environmental Medicine, University of North Carolina at Chapel Hill, ⁴Department of Cell Biology & Physiology, University of North Carolina at Chapel Hill, ⁵McAllister Heart Institute, University of North Carolina at Chapel Hill

JoVE 60473

In Vitro and In Vivo Delivery of Magnetic Nanoparticle Hyperthermia using a Custom-Built Delivery System

Kayla E. A. Duval¹, James D. Petryk², P. Jack Hoopes^1,2

¹Thayer School of Engineering, Dartmouth College, ²Geisel School of Medicine, Dartmouth College

JoVE 61413

Single Myofiber Isolation and Culture from a Murine Model of Emery-Dreifuss Muscular Dystrophy in Early Post-Natal Development

Gloria Pegoli^1,2, Federica Lucini^1,2, Chiara Mozzetta³, Chiara Lanzuolo^1,2,4

¹Istituto Nazionale di Genetica Molecolare "Romeo ed Enrica Invernizzi", ²IRCCS Santa Lucia Foundation, ³Department of Biology and Biotechnology, "C. Darwin" Sapienza University, CNR - IBPM, ⁴CNR – Institute of Biomedical Technologies (ITB)

JoVE 61516

Microscopic Observation of Lymphocyte Dynamics in Rat Peyer's Patches

Kazuhiko Shirakabe^1,2, Masaaki Higashiyama¹, Naoki Shibuya², Kazuki Horiuchi¹, Masayuki Saruta², Ryota Hokari¹

¹Department of internal medicine, National Defense Medical College, ²Division of Gastroenterology and Hepatology, Department of Internal Medicine, Jikei University School of Medicine

JoVE 61568

A Murine Model of Fetal Exposure to Maternal Inflammation to Study the Effects of Acute Chorioamnionitis on Newborn Intestinal Development

Brian A. Juber¹, Timothy G. Elgin¹, Erin M. Fricke², Huyiu Gong¹, Jeffrey Reese³, Steven J. McElroy^1,4

¹Division of Neonatology, Stead Family Department of Pediatrics, University of Iowa, ²Division of Maternal Fetal Medicine, Department of Obstetrics & Gynecology, University of Iowa, ³Division of Neonatology, Department of Pediatrics, Vanderbilt University, ⁴Department of Microbiology & Immunology, University of Iowa

JoVE 61464

In vivo Mouse Model of Spinal Implant Infection

Benjamin V. Kelley¹, Stephen D. Zoller¹, Danielle Greig¹, Kellyn Hori¹, Nicolas Cevallos¹, Chad Ishmael¹, Peter Hsiue¹, Rishi Trikha¹, Troy Sekimura², Thomas Olson², Ameen Chaudry², Michael M. Le², Anthony A. Scaduto¹, Kevin P. Francis¹, Nicholas M. Bernthal¹

¹Department of Orthopaedic Surgery, University of California Los Angeles, ²David Geffen School of Medicine, University of California Los Angeles

JoVE 60560

In Vitro Assessment of Cardiac Function Using Skinned Cardiomyocytes

Patrícia Gonçalves-Rodrigues*¹, João Almeida-Coelho*¹, Alexandre Gonçalves¹, Flávio Amorim¹, Adelino F. Leite-Moreira¹, Ger J.M. Stienen², Inês Falcão-Pires¹

¹Unidade de Investigação Cardiovascular, Departamento de Cirurgia e Fisiologia, Faculdade de Medicina, Universidade do Porto, ²Department of Physiology, Kilimanjaro Christian Medical University College

JoVE 60427

Electrocardiogram Recordings in Anesthetized Mice using Lead II

Tae Woong Ha¹, Bermseok Oh*¹, Ji-One Kang*¹

¹Department of Biochemistry and Molecular Biology, School of Medicine, Kyung Hee University

JoVE 61583

Measurement of the Hepatic Venous Pressure Gradient and Transjugular Liver Biopsy

Thomas Reiberger^1,2, Philipp Schwabl^1,2, Michael Trauner^1,2, Markus Peck-Radosavljevic^2,3, Mattias Mandorfer^1,2

¹Division of Gastroenterology & Hepatology, Department of Internal Medicine III, Medical University of Vienna, ²Vienna Hepatic Hemodynamic Lab, Medical University of Vienna, ³Department of Internal Medicine and Gastroenterology (IMuG), Klinikum Klagenfurt am Woerthersee

JoVE 58819

Evaluation of Color Difference in Placenta with Twin Anemia Polycythemia Sequence

Depeng Zhao¹, Lisanne S.A. Tollenaar², Femke Slaghekke², Dick Oepkes², Tao Duan³, Enrico Lopriore⁴

¹Department of Reproductive Medicine, Affiliated Shenzhen Maternity and Child Healthcare Hospital, Southern Medical University, ²Division of Fetal Medicine, Department of Obstetrics, Leiden University Medical Center, ³Fetal Medicine Unit & Prenatal Diagnosis Center, Department of Obstetrics, Shanghai First Maternity and Infant Hospital, Tongji University School of Medicine, ⁴Division of Neonatology, Department of Pediatrics, Leiden University Medical Center

JoVE 61312

Using a Chemical Biopsy for Graft Quality Assessment

Iga Stryjak*¹, Natalia Warmuzińska*¹, Kamil Łuczykowski¹, Matyas Hamar², Peter Urbanellis², Emilia Wojtal³, Marek Masztalerz³, Markus Selzner^2,4, Zbigniew Włodarczyk³, Barbara Bojko¹

¹Department of Pharmacodynamics and Molecular Pharmacology, Faculty of Pharmacy, Nicolaus Copernicus University in Torun, Collegium Medicum in Bydgoszcz, ²Multi Organ Transplant Program, Department of Surgery, Toronto General Hospital, University Health Network, ³Department of Transplantation and General Surgery, University Hospital, University of Nicolaus Copernicus Torun, ⁴Department of Medicine, Toronto General Hospital