JoVE Journal

Medicine

JoVE Medicine includes methods and experimental approaches for biomedical research and clinical medicine, including case studies, clinical procedures, surgeries, clinical trials, and animal models of disease.

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Robotic Left Hepatectomy using Indocyanine Green Fluorescence Imaging for an Intrahepatic Complex Biliary Cyst

Burak Görgec^1,2, Babs M. Zonderhuis^2,3, Marc G. Besselink^1,2, Joris Erdmann^1,2,3, Geert Kazemier^2,3, Rutger-Jan Swijnenburg^1,2,3

¹Department of Surgery, Amsterdam UMC, location University of Amsterdam, ²Cancer Center Amsterdam, ³Department of Surgery, Amsterdam UMC, location Vrije Universiteit

JoVE 63265

Methods Collection

Determining Binding Affinity (K_D) of Radiolabeled Antibodies to Immobilized Antigens

Erika Belitzky¹, Alessandra Cavaliere¹, Khashayar Rajabimoghadam¹, Bernadette Marquez-Nostra¹

¹Department of Radiology and Biomedical Imaging, Yale University

JoVE 63927

Methods Collection

Standardized Rat Coronary Ring Preparation and Real-Time Recording of Dynamic Tension Changes Along Vessel Diameter

Pengmei Guo*¹, Wenqiao An*², Ying Guo¹, Zhihao Sun², Xiaobo Wang^1,3, Sanyin Zhang^1,3

¹Innovative Institute of Chinese Medicine and Pharmacy, Chengdu University of Traditional Chinese Medicine, ²School of Basic Medicine, Chengdu University of Traditional Chinese Medicine, ³Research Institute of Integrated TCM & Western Medicine, Chengdu University of Traditional Chinese Medicine

JoVE 64121

Testing Epithelial Permeability in Fetal Tissue-Derived Enteroids

Amelia Llerena¹, Shaheda Urmi², Jahanshah Amin³, Byeong Cha³, Thao TB Ho²

¹Morsani College of Medicine, University of South Florida, ²Department of Pediatrics, Morsani College of Medicine, University of South Florida, ³Department of Molecular Pharmacology and Physiology, Morsani College of Medicine, University of South Florida

JoVE 64108

Methods Collection

Robotic Cochlear Implantation for Direct Cochlear Access

Marco Caversaccio^1,2, Georgios Mantokoudis¹, Franca Wagner³, Philipp Aebischer^1,2, Stefan Weder^1,2, Wilhelm Wimmer^1,2

¹Department of Otorhinolaryngology, Head and Neck Surgery, Inselspital, Bern University Hospital, University of Bern, ²Hearing Research Laboratory, ARTORG Center for Biomedical Engineering Research, University of Bern, ³Department of Diagnostic and Interventional Neuroradiology, Inselspital, Bern University Hospital

JoVE 64047

Methods Collection

A Simple Pit Assay Protocol to Visualize and Quantify Osteoclastic Resorption In Vitro

Wanjing Cen¹, Siegmar Reinert¹, Meltem Avci-Adali², Dorothea Alexander¹, Felix Umrath^1,3

¹Department of Oral and Maxillofacial Surgery, University Hospital Tübingen, ²Department of Thoracic and Cardiovascular Surgery, University Hospital Tübingen, ³Department of Orthopaedic Surgery, University Hospital Tübingen

JoVE 64016

Methods Collection

Dissection Techniques and Histological Sampling of the Heart in Large Animal Models for Cardiovascular Diseases

Irina Constantin¹, Alexandru Flaviu Tăbăran¹

¹Department of Pathology, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca

JoVE 63809

Methods Collection

Transcorporal Artificial Urinary Sphincter Cuff Placement in a Case Requiring Revision for Urethral Atrophy

Da-chao Zheng*¹, Min-kai Xie*¹, Jie-wen Bao¹, Hai-jun Yao¹, Zhong Wang¹

¹Department of Urology, Shanghai 9th People’s Hospital, Shanghai Jiaotong University School of Medicine

JoVE 63678

Methods Collection

Rectal Organoid Morphology Analysis (ROMA): A Diagnostic Assay in Cystic Fibrosis

Senne Cuyx^1,2, Anabela S. Ramalho¹, Nikky Corthout^3,4, Steffen Fieuws⁵, Eva Fürstová⁶, Kaline Arnauts^7,8, Marc Ferrante^7,9, Catherine Verfaillie⁸, Sebastian Munck^3,4, Mieke Boon^1,2, Marijke Proesmans^1,2, Lieven Dupont^10,11, Kris De Boeck^1,2, François Vermeulen^1,2

¹Department of Development and Regeneration, Woman and Child Unit, CF research lab, KU Leuven, ²Department of Pediatrics, Pediatric Pulmonology, University Hospitals Leuven, ³VIB Bio Imaging Core, VIB-KU Leuven Center for Brain & Disease Research, ⁴Department for Neuroscience, KU Leuven, ⁵Interuniversity Center for Biostatistics and Statistical Bioinformatics, University of Leuven and University of Hasselt, ⁶Department of Pediatrics, 2^nd Faculty of Medicine, Charles University and Motol University Hospital, ⁷Department of Chronic Diseases and Metabolism (CHROMETA), Translational Research Center for Gastrointestinal Disorders (TARGID), KU Leuven, ⁸Department of Development and Regeneration, Stem Cell Institute Leuven (SCIL), KU Leuven, ⁹Department of Gastroenterology and Hepatology, University Hospitals Leuven, KU Leuven, ¹⁰Department of Chronic Diseases, Metabolism and Ageing; Pneumology, KU Leuven, ¹¹Department of Respiratory Diseases, University Hospitals Leuven

JoVE 63818

Methods Collection

In Vitro Apical-Out Enteroid Model of Necrotizing Enterocolitis

Kathryn Burge¹, Adam Wilson¹, Hala Chaaban¹

¹Department of Pediatrics, Division of Neonatal-Perinatal Medicine, University of Oklahoma Health Sciences Center and Center for Pregnancy and Newborn Health

JoVE 64003

Methods Collection

A Modified Vessel-sparing Microsurgical Vasoepididymostomy

Liang Zhou¹, Xingzhe Ji¹, Lei Wang¹

¹Assisted Reproduction Center, Northwest Women’s and Children’s Hospital

JoVE 63894

Methods Collection

Fiber Type and Subcellular-Specific Analysis of Lipid Droplet Content in Skeletal Muscle

Camille M. Selvais¹, Laura L. De Cock¹, Sonia M. Brichard¹, María A. Davis-López de Carrizosa^1,2

¹Endocrinology, Diabetes and Nutrition Unit, Institute of Experimental and Clinical Research, Medical Sector, Université Catholique de Louvain, ²Departamento de Fisiología, Facultad de Biología, Universidad de Sevilla

JoVE 63718

Methods Collection

Isolation of Intrapulmonary Artery and Smooth Muscle Cells to Investigate Vascular Responses

Kittiwoot To-on¹, Usana Chatturong¹, Teerapap Panklai², Iyapa Palang¹, Anjaree Inchan¹, Sutthinee Wisutthathum³, Tamkeen Urooj Paracha⁴, Phapada Apaikawee¹, Krongkarn Chootip¹

¹Department of Physiology, Faculty of Medical Science and Center of Excellence for Innovation in Chemistry, Naresuan University, ²Department of Pharmaceutical Chemistry and Pharmacognosy, Faculty of Pharmaceutical Sciences, Naresuan University, ³Cosmetics and Natural Products Research Center, Faculty of Pharmaceutical Sciences, Naresuan University, ⁴Snyder Institute for Chronic Diseases, Airways Inflammation Research Group, Department of Physiology and Pharmacology, University of Calgary

JoVE 63686

Methods Collection

Transcatheter Pulmonary Valve Replacement from Autologous Pericardium with a Self-Expandable Nitinol Stent in an Adult Sheep Model

Yimeng Hao^1,2, Xiaolin Sun^1,2, Jonathan Frederik Sebastian Kiekenap¹, Jasper Emeis¹, Marvin Steitz², Alexander Breitenstein-Attach², Felix Berger^1,2, Boris Schmitt^1,2,3,4,5

¹Department of Pediatric Cardiology and Congenital Heart Disease, Charité University Medicine Berlin, ²Department of Pediatric Cardiology and Congenital Heart Disease, Deutsches Herzzentrum Berlin, ³DZHK (German Centre for Cardiovascular Research) and BMBF (German Ministry of Education and Research), ⁴BIH (Berlin Institute of Health), ⁵BCRT (BIH Center of Regenerative Therapies)