JoVE Journal

Medicine

JoVE Medicine includes methods and experimental approaches for biomedical research and clinical medicine, including case studies, clinical procedures, surgeries, clinical trials, and animal models of disease.

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Retinal Pigment Epithelium Transplantation in a Non-human Primate Model for Degenerative Retinal Diseases

Ivan Seah*¹, Zengping Liu*^2,3,4, Daniel Soo Lin Wong³, Wendy Wong¹, Graham E. Holder^1,3,5, Veluchamy Amutha Barathi^3,4,6, Gopal Lingam^1,3,4, Xinyi Su^1,2,3,4, Boris V. Stanzel^1,7,8

¹Department of Ophthalmology, National University Hospital, Singapore, ²Institute of Molecular and Cell Biology (IMCB), Agency for Science, Technology and Research (A*STAR), ³Department of Ophthalmology, Yong Loo Lin School of Medicine, National University of Singapore, ⁴Singapore Eye Research Institute (SERI), ⁵UCL Institute of Ophthalmology, ⁶Academic Clinical Program in Ophthalmology, Duke-NUS Medical School, ⁷Macula Center Saar, Eye Clinic Sulzbach, Knappschaft Hospital Saar, ⁸Department of Ophthalmology, University of Bonn

JoVE 62638

Methods Collection

Utilizing Percutaneous Ventricular Assist Devices in Acute Myocardial Infarction Complicated by Cardiogenic Shock

Shuktika Nandkeolyar¹, Poonam Velagapudi², Mir B. Basir³, Aditya S. Bharadwaj¹

¹Division of Cardiology, Loma Linda University Medical Center, ²Division of Cardiology, University of Nebraska, ³Division of Cardiology, Henry Ford Health System

JoVE 62110

Methods Collection

A New Model of Heart Failure Post-Myocardial Infarction in the Rat

Honglei Li^1,2, Jiansheng Huang³, Caihong Liu¹, Zhentao Zhang³, Kuiquan Song², Ke Ma¹, Connor W. Dennewitz³, Shijun Wang^1,4

¹College of Traditional Chinese Medicine, Shandong University of Traditional Chinese Medicine, ²Department of Intensive Care Unit, Jinan Municipal Hospital of Traditional Chinese Medicine, ³Department of Medicine, Vanderbilt University Medical Center, ⁴Shandong Co-Innovation Center of Classic TCM Formula, Shandong Provincial Education Department

JoVE 62540

Functional Characterization of Endogenously Expressed Human RYR1 Variants

Susan Treves^1,2, Thierry Girard³, Francesco Zorzato^1,2

¹Department of Biomedicine, Basel University Hospital, ²Department of Life Sciences, University of Ferrara, ³Department of Anesthesia, Basel University Hospital

JoVE 62196

Methods Collection

Self-Administration of Drugs in Mouse Models of Feeding and Obesity

Rizaldy C. Zapata¹, Dinghong Zhang¹, Besma Chaudry¹, Olivia Osborn¹

¹Division of Endocrinology and Metabolism, Department of Medicine, University of California San Diego

JoVE 62775

An In Vivo Mouse Model of Total Intravenous Anesthesia during Cancer Resection Surgery

Julia A. Dubowitz^1,2,3, Fabian Jost-Brinkmann^1,4,5, Alexandra I. Ziegler¹, Ryan D. Gillis¹, Bernhard Riedel^1,2,3,6, Erica K. Sloan^1,2

¹Drug Discovery Biology Theme, Monash Institute of Pharmaceutical Sciences, Monash University, ²Department of Anaesthesia, Division of Cancer Surgery, Peter MacCallum Cancer Centre, ³Department of Critical Care, Melbourne Medical School, University of Melbourne, ⁴Medical Department, Division of Hepatology and Gastroenterology, Charité - Universitätsmedizin Berlin, ⁵Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, ⁶Sir Peter MacCallum Department of Oncology, University of Melbourne

JoVE 62747

The Use of 3D Echocardiography in Surgical Planning of the Mitral Valve in Pediatric Cardiology

Nick Arbic¹, Andreea Dragulescu¹, Luc Mertens¹, Olivier Villemain¹

¹Division of Cardiology, Department of Pediatrics, The Hospital for Sick Children, University of Toronto

JoVE 62574

Methods Collection

Blood Circuit Reconstruction in an Abdominal Mouse Heart Transplantation Model

Decheng Yin*^1,2, Jian Fu*^1,3, Ida Allabauer¹, Oliver Witzke⁴, Song Rong⁵, André Hoerning¹

¹Department of Pediatric and Adolescent Medicine, University Hospital Erlangen, Friedrich-Alexander University Erlangen-Nürnberg, ²Department of Pediatric Surgery, Chengdu Women's and Children's Central Hospital, ³The Key Laboratory of Hainan Trauma and Disaster Rescue, The first affiliated Hospital of Hainan Medical University, ⁴Department of Infectious Diseases, West German Centre of Infectious Diseases, Universitätsmedizin Essen, University Duisburg-Essen, ⁵Department of Nephrology, Hannover Medical School

JoVE 62007

Basophil Activation Test for Allergy Diagnosis

Inmaculada Doña*^1,2, Adriana Ariza*², Tahia D. Fernández^2,3, María J. Torres^1,2,4,5

¹Allergy Unit, Hospital Regional Universitario de Málaga, ²Allergy Research Group, Instituto de Investigación Biomédica de Málaga-IBIMA, ³Universidad de Málaga, Departamento de Biología celular, Genética y Fisiología, Universidad de Málaga, ⁴Departamento de Medicina, Universidad de Málaga, ⁵Nanostructures for Diagnosing and Treatment of Allergic Diseases Laboratory, Andalusian Center for Nanomedicine and Biotechnology-BIONAND

JoVE 62600

Visualizing Membrane Ruffle Formation using Scanning Electron Microscopy

WonMo Ahn¹, Bhupesh Singla¹, Brendan Marshall², Gábor Csányi^1,3

¹Vascular Biology Center, Medical College of Georgia at Augusta University, ²Department of Cellular Biology and Anatomy, Medical College of Georgia at Augusta University, ³Department of Pharmacology and Toxicology, Medical College of Georgia at Augusta University

JoVE 62658

Methods Collection

Fat-Covered Islet Transplantation using Epididymal White Adipose Tissue

Naoaki Sakata^1,2, Gumpei Yoshimatsu^1,2, Ryo Kawakami^1,2, Shohta Kodama^1,2

¹Department of Regenerative Medicine and Transplantation, Faculty of Medicine, Fukuoka University, ²Center for Regenerative Medicine, Fukuoka University Hospital

JoVE 62096

Precision Cut Lung Slices as an Efficient Tool for Ex vivo Pulmonary Vessel Structure and Contractility Studies

Timothy Klouda¹, Hyunbum Kim¹, Jiwon Kim¹, Gary Visner¹, Ke Yuan¹

¹Divisions of Pulmonary Medicine, Boston Children's Hospital

JoVE 62392

Methods Collection

Endaural Endoscopic Atticoantrotomy (Retrograde Mastoidectomy) using a Constant Suction Bone-drilling Technique

Zhen Gao*^1,2,3, Xian-hao Jia*^1,2,3, Ya-sheng Yuan^1,2,3

¹Department of Otology and Skull Base Surgery, Eye & ENT Hospital, Fudan University, ²Shanghai Auditory Medical Center, ³NHC Key Laboratory of Hearing Medicine (Fudan University)

JoVE 62450

Analyzing Long-Term Electrocardiography Recordings to Detect Arrhythmias in Mice

Philipp Tomsits^1,2,3, Kavi Raj Chataut^1,2, Aparna Sharma Chivukula^1,2, Li Mo^1,2, Ruibing Xia^1,2, Dominik Schüttler^1,2,3, Sebastian Clauss^1,2,3

¹Department of Medicine I, University Hospital Munich, Campus Großhadern, Ludwig-Maximilians University Munich (LMU), ²DZHK (German Centre for Cardiovascular Research), Partner Site Munich, Munich Heart Alliance (MHA), ³Walter Brendel Centre of Experimental Medicine, Ludwig-Maximilians University Munich (LMU)

JoVE 62386

Methods Collection

Diagnosis and Surgical Treatment of Human Brucellar Spondylodiscitis

Yakefu Abulizi*¹, Xiaoyu Cai*^1,2,3, Tao Xu¹, Chuanhui Xun¹, Weibin Sheng¹, Liang Gao^2,3, Maierdan Maimaiti¹

¹Department of Spine Surgery, First Affiliated Hospital of Xinjiang Medical University, ²Center of Experimental Orthopaedics, Saarland University Medical Center, ³Sino Euro Orthopaedics Network (SEON)

JoVE 61840

Methods Collection

Cardiac Response to β-Adrenergic Stimulation Determined by Pressure-Volume Loop Analysis

Rebekka Medert*^1,2, Lucas Bacmeister*^1,2,3, Sebastian Segin*^1,2,4, Marc Freichel^1,2, Juan E. Camacho Londoño^1,2

¹Pharmakologisches Institut, Ruprecht-Karls-Universität Heidelberg, ²DZHK (German Centre for Cardiovascular Research), partner site Heidelberg/Mannheim, ³Department of Internal Medicine III, University of Heidelberg, ⁴Department of Anesthesiology, University Hospital RWTH Aachen

JoVE 62057

Closed Chest Biventricular Pressure-Volume Loop Recordings with Admittance Catheters in a Porcine Model

Mads Dam Lyhne^1,2, Jacob Gammelgaard Schultz^1,2, Simone Juel Dragsbaek^1,2, Jacob Valentin Hansen^1,2, Christian Schmidt Mortensen^1,2, Anders Kramer^1,2, Jens Erik Nielsen-Kudsk^1,2, Asger Andersen^1,2

¹Department of Cardiology Research, Aarhus University Hospital, ²Department of Clinical Medicine, Aarhus University

JoVE 62661

A Model of Experimental Steatosis In Vitro: Hepatocyte Cell Culture in Lipid Overload-Conditioned Medium

Adriana Campos-Espinosa¹, Carolina Guzmán¹

¹Laboratorio de Hígado, Páncreas y Motilidad, Unidad de Medicina Experimental, Facultad de Medicina-UNAM, Hospital General de México “Dr. Eduardo Liceaga”

JoVE 62543

Methods Collection

Refined CLARITY-Based Tissue Clearing for Three-Dimensional Fibroblast Organization in Healthy and Injured Mouse Hearts

Demetria M. Fischesser^1,2, Evan C. Meyer³, Michelle Sargent², Jeffery D. Molkentin²

¹Department of Molecular Genetics, Biochemistry, and Microbiology, University of Cincinnati College of Medicine, ²Division of Molecular Cardiovascular Biology, Cincinnati Children's Hospital Medical Center, ³Confocal Imaging Core, Cincinnati Children's Hospital Medical Center

JoVE 62023

Acute Kidney Injury Model Induced by Cisplatin in Adult Zebrafish

Camila Morales Fénero*¹, Barbara Nunes Padovani*¹, Mariana Abrantes do Amaral*^1,2, Guilherme José Bottura de Barros¹, Izabella Karina Xavier de Oliveira¹, Meire Ioshie Hiyane¹, Niels Olsen Saraiva Camâra^1,2

¹Department of Immunology, University of São Paulo, ²Department of Medicine, Nephrology Division, Federal University of São Paulo

JoVE 61575

Methods Collection

Modifications of the Langendorff Method for Simultaneous Isolation of Atrial and Ventricular Myocytes from Adult Mice

Kui Wu¹, Lin-Ling Li², Yu-Kun Li¹, Xiao-Dong Peng¹, Meng-Xia Zhang¹, Ke-Sen Liu¹, Xue-Si Wang¹, Jia-Xue Yang¹, Song-Nan Wen¹, Yan-Fei Ruan¹, Nian Liu¹, Rong Bai¹

¹Department of Cardiology, Bejing Anzhen Hospital, Capital Medical University, ²Department of Cardiology, Bejing Chuiyangliu Hospital

JoVE 62514

Intra-Abdominal Pressure Measurements in Neurocritical Patients

Xuxu Xu*¹, Zhenghui He*¹, Yingchi Shan², Qing Mao¹, Junfeng Feng¹, Guoyi Gao²

¹Department of Neurosurgery, Renji Hospital, Shanghai Jiaotong University School of Medicine, ²Department of Neurosurgery, Shanghai General Hospital, Shanghai Jiaotong University School of Medicine

JoVE 62557

Measuring Cardiac Output in a Swine Model

Neerav Patel¹, Hossam Abdou¹, Joseph Edwards¹, Noha N. Elansary¹, Kelly Poe¹, Michael J. Richmond¹, Marta J. Madurska¹, Todd E. Rasmussen², Jonathan J. Morrison¹

¹R Adams Cowley Shock Trauma Center, University of Maryland Medical System, ²Uniformed Services University of the Health Sciences

JoVE 62333

Minimizing Post-Infusion Portal Vein Bleeding during Intrahepatic Islet Transplantation in Mice

Wenyu Gou*¹, Wanxing Cui*², Yuki Cui², Hongjun Wang^1,3

¹Department of Surgery, Medical University of South Carolina, ²Georgetown University, ³Ralph H. Johnson Veterans Affairs Medical Center

JoVE 62530

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Bioengineering

Biology

Cancer Research

Immunology and Infection

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