JoVE Journal

Medicine

JoVE Medicine includes methods and experimental approaches for biomedical research and clinical medicine, including case studies, clinical procedures, surgeries, clinical trials, and animal models of disease.

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Halogenated Agent Delivery in Porcine Model of Acute Respiratory Distress Syndrome via an Intensive Care Unit Type Device

Raiko Blondonnet^1,2, Bertille Paquette^1,2, Jules Audard^1,2, Ridvan Guler^1,2, François-Xavier Roman^1,2, Ruoyang Zhai², Corinne Belville², Loïc Blanchon², Thomas Godet¹, Emmanuel Futier^1,2, Jean-Etienne Bazin¹, Jean-Michel Constantin³, Vincent Sapin^2,4, Matthieu Jabaudon^1,2,5

¹Department of Perioperative Medicine, CHU Clermont-Ferrand, ²GReD, CNRS, INSERM, Université Clermont Auvergne, ³GRC 29, AP-HP, DMU DREAM, Department of Anesthesiology and Critical Care, Pitié-Salpêtrière Hospital, Sorbonne University, ⁴Department of Biochemistry and Molecular Genetics, CHU Clermont-Ferrand, ⁵Division of Allergy, Pulmonary, and Critical Care Medicine, Vanderbilt University Medical Center

JoVE 61644

Predicting Amputation using Local Circulating Mononuclear Progenitor Cells in Angioplasty-treated Patients with Critical Limb Ischemia

Juan Antonio Suárez-Cuenca¹, Eduardo Vera-Gómez¹, Alejandro Hernández-Patricio¹, Atzín Suá Ruíz-Hernández¹, Juan Ariel Gutiérrez-Buendía¹, Carlos Ramiro Zamora-Alemán¹, Alberto Melchor-López¹, Yasser Alberto Rizo-García², Oscar Antonio Lomán-Zúñiga², Ignacio Escotto-Sánchez², Juan Miguel Rodríguez-Trejo², Rebeca Pérez-Cabeza de Vaca³, Mario Antonio Téllez-González³, Paul Mondragón-Terán³

¹Experimental Metabolism and Clinical Research Laboratory, Clinical Research Department, Division of Biomedical Research, Centro Médico Nacional 20 de Noviembre, ISSSTE, ²Vascular Surgery and Angiology Department, Centro Médico Nacional 20 de Noviembre, ISSSTE, ³Regenerative Medicine and Tissue Engineering Laboratory; Coordination of Research, Centro Médico Nacional 20 de Noviembre, ISSSTE

JoVE 61503

Methods Collection

Intramyocardial Transplantation of MSC-Loading Injectable Hydrogels after Myocardial Infarction in a Murine Model

Chan Woo Kim^1,2, Chan Joon Kim^1,3, Eun-Hye Park^1,2, Eunmi Lee^1,2, Eunhwa Seong^1,2, Kiyuk Chang^1,2

¹Cardiovascular Research Institute for Intractable Disease, College of Medicine, The Catholic University of Korea, ²Division of Cardiology, Department of Internal Medicine, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, ³Division of Cardiology, Department of Internal Medicine, Uijeongbu St. Mary's Hospital, College of Medicine, The Catholic University of Korea

JoVE 61752

Oxygen-Induced Retinopathy Model for Ischemic Retinal Diseases in Rodents

Maria Vähätupa^1,2, Niina Jääskeläinen², Marc Cerrada-Gimenez², Rubina Thapa², Tero Järvinen¹, Giedrius Kalesnykas², Hannele Uusitalo-Järvinen^1,3

¹Faculty of Medicine and Health Technology, Tampere University & Tampere University Hospital, ²Experimentica Ltd, ³Eye Centre, Tampere University Hospital

JoVE 61482

The Lublin Protocol of the Uterine Arteries Embolization in the Treatment of Symptomatic Uterine Fibroids

Piotr Szkodziak*¹, Krzysztof Pyra*², Filip Szkodziak¹, Jarosław Krzyżanowski¹, Piotr Czuczwar¹, Sławomir Woźniak¹, Tomasz Jargiełło², Tomasz Paszkowski¹

¹3rd Chair and Department of Gynecology, Medical University of Lublin, ²Department of Interventional Radiology and Neuroradiology, Medical University of Lublin

JoVE 61530

Transaxillary First Rib Resection for Treatment of the Thoracic Outlet Syndrome

Murat Akkuş¹, Selcuk Kose², Yaşar Sönmezoğlu³

¹Department of Thoracic Surgery, Mehmet Akif Ersoy Thoracic and Cardiovascular Surgery Training and Research Hospital, ²Department of Thoracic Surgery, Bakırköy Dr. Sadi Konuk Training and Research Hospital, ³Department of Thoracic Surgery, Yedikule Chest Disease and Thoracic Surgery Training and Research Hospital

JoVE 59659

A Quantitative Detection Method for MicroRNAs in the Kidney of an Ischemic Kidney Injury Mouse Model

Akinori Aomatsu¹, Keiji Hirai¹, Hiroki Ishii¹, Katsunori Yanai¹, Shohei Kaneko¹, Yoshiyuki Morishita¹

¹Division of Nephrology, First Department of Integrated Medicine, Saitama Medical Center, Jichi Medical University

JoVE 61378

Methods Collection

Operating Transverse Aortic Constriction with Absorbable Suture to Obtain Transient Myocardial Hypertrophy

Yi Lao¹, Cankun Zheng¹, Hailin Zhu^1,2, Hairuo Lin¹, Xiaoxia Huang¹, Yulin Liao¹

¹Department of Cardiology, State Key Laboratory of Organ Failure Research, Nanfang Hospital, Southern Medical University, ²Department of Geriatrics, Pingxiang People's Hospital of Southern Medical University

JoVE 61686

Electroretinogram Recording for Infants and Children under Anesthesia to Achieve Optimal Dark Adaptation and International Standards

Byron L. Lam¹, Carlos Mendoza-Santiestaban¹, Alex Gonzalez^1,2, Cornelis Rowaan^1,2, Mu Liu¹, Joanne Martin¹, Steven Gayer^1,3, Osmany Gil Figueredo¹, Jean-Marie Parel^1,2

¹Department of Ophthalmology, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, ²Ophthalmic Biophysics Center, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, ³Department of Anesthesiology, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine

JoVE 61734

Delayed Intramyocardial Delivery of Stem Cells after Ischemia Reperfusion Injury in a Murine Model

Michaela Olthoff¹, Federico Franchi¹, Karen M. Peterson¹, Ramasamy Paulmurugan², Martin Rodriguez-Porcel¹

¹Department of Cardiovascular Medicine, Mayo Clinic, ²Department of Radiology and Molecular Imaging Program at Stanford (MIPS), Stanford University School of Medicine

JoVE 61546

Taking the Next Step: a Neural Coaptation Orthotopic Hind Limb Transplant Model to Maximize Functional Recovery in Rat

Feibo Zheng^1,3, Andy Tully^1,2, Kyle M Koss¹, Xiaomin Zhang¹, Longhui Qiu¹, Jiao-Jing Wang¹, Bilal A Naved^1,4, David Z Ivancic¹, James M Mathew¹, Jason A Wertheim¹, Zheng Jenny Zhang¹

¹Comprehensive Transplant Center and Department of Surgery, Feinberg School of Medicine, Northwestern University, ²Department of Surgery, University of Illinois at Chicago, ³Department of Surgery, Tianjin Occupational Diseases Precaution and Therapeutic Hospital, ⁴Department of Biomedical Engineering, McCormick School of Engineering, Northwestern University

JoVE 60777

Acupuncture in a Rat Model of Asthma

Dong-Dong Zhou¹, Guang-Quan Zhang¹, Qing-Yi Zhao¹, Mi Cheng¹, Jin Lu¹, Yu Wang¹, Yu-Dong Xu¹, Yan-Jiao Chen¹, Yong-Qing Yang¹, Lei-Miao Yin^1,2

¹Laboratory of Molecular Biology, Shanghai Research Institute of Acupuncture and Meridian, Shanghai University of Traditional Chinese Medicine, ²Shanghai Innovation Center of Traditional Chinese Medicine Health Service

JoVE 61762

Development of a Selective Aortic Arch Perfusion System in a Porcine Model of Exsanguination Cardiac Arrest

Marta J. Madurska¹, Hossam Abdou¹, Michael J. Richmond¹, Noha N. Elansary¹, Peng F. Wong², Todd E. Rasmussen³, Thomas M. Scalea¹, Jonathan J. Morrison¹

¹R Adams Cowley Shock Trauma Center, ²Department of Vascular Surgery, The James Cook University Hospital, ³Uniformed Services University of the Health Sciences

JoVE 61573

Isolation and Cultivation of Mandibular Bone Marrow Mesenchymal Stem Cells in Rats

Yueyang Hong*¹, Hongyuan Xu*¹, Yiling Yang¹, Siru Zhou¹, Anting Jin¹, Xiangru Huang¹, Qinggang Dai², Lingyong Jiang¹

¹Center of Craniofacial Orthodontics, Department of Oral and Cranio-maxillofacial Science, Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, National Clinical Research center of Stomatology, ²The 2nd Dental Center, Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, National Clinical Research center of Stomatology

JoVE 61532

Induction of Accelerated Atherosclerosis in Mice: The "Wire-Injury" Model

Adelina Curaj¹, Wu Zhoujun¹, Mareike Staudt¹, Elisa A. Liehn^1,2

¹Institute for Molecular Cardiovascular Research, RWTH Aachen University, ²Human Genetic Laboratory, University of Medicine and Pharmacy

JoVE 54571

Orthotopic Kidney Auto-Transplantation in a Porcine Model Using 24 Hours Organ Preservation And Continuous Telemetry

Wen-Jia Liu*^1,2, Lisa Ernst*², Benedict Doorschodt², Jan Bednarsch¹, Felix Becker³, Richi Nakatake², Yuki Masano², Ulf Peter Neumann^1,4, Sven Arke Lang¹, Peter Boor⁵, Isabella Lurje⁶, Georg Lurje^1,6, René Tolba², Zoltan Czigany^1,2

¹Department of Surgery and Transplantation, Faculty of Medicine, University Hospital RWTH Aachen, ²Institute for Laboratory Animal Science, Faculty of Medicine, University Hospital RWTH Aachen, ³Department of General-, Visceral-, and Transplantation Surgery, University Hospital Muenster, ⁴Department of Surgery, Maastricht University Medical Centre (MUMC), ⁵Institute of Pathology, Faculty of Medicine, University Hospital RWTH Aachen, ⁶Department of Surgery, Campus Charité Mitte | Campus Virchow-Klinikum, Charité-Universitätsmedizin

JoVE 61591

An In Vitro Hemodynamic Loop Model to Investigate the Hemocytocompatibility and Host Cell Activation of Vascular Medical Devices

Max Wacker¹, Ulf Betke², Katrin Borucki³, Jörn Hülsmann¹, George Awad¹, Sam Varghese¹, Maximilian Scherner¹, Michael Hansen⁴, Jens Wippermann¹, Priya Veluswamy¹

¹Department of Cardiothoracic Surgery, Otto-von-Guericke-University, ²Department of Mechanical Engineering, Institute for Materials and Joining Technology, Otto-von-Guericke-University, ³Institute of Clinical Chemistry and Pathobiochemistry, Otto-von-Guericke-University, ⁴Division of Cardiology and Angiology, Department of Internal Medicine, Otto-von-Guericke-University

JoVE 61570

How to Administer Near-Infrared Spectroscopy in Critically ill Neonates, Infants, and Children

Nora Bruns¹, Julia Moosmann², Frank Münch³, Christian Dohna-Schwake¹, Joachim Woelfle⁴, Robert Cesnjevar³, Sven Dittrich², Ursula Felderhoff-Müser¹, Hanna Müller⁴

¹Department of Pediatrics I - Neonatology, Pediatric Intensive Care, Pediatric Neurology, University Hospital Essen, University of Duisburg-Essen, ²Department of Pediatric Cardiology, University Hospital Erlangen, University of Erlangen-Nürnberg, ³Department of Pediatric Cardiac Surgery, University Hospital Erlangen, University of Erlangen-Nürnberg, ⁴Division of Neonatology and Pediatric Intensive Care, Department of Pediatrics, University Hospital Erlangen, University of Erlangen-Nürnberg

JoVE 61533

Establishing In Situ Closed Circuit Perfusion of Lower Abdominal Organs and Hind Limbs in Mice

Ping Ren^1,2,3, Chunyan Yang^2,4, Laren A. Lofchy², Guankui Wang², Fangfang Chen*^2,4,5, Dmitri Simberg*²

¹Department of Thoracic Surgery, The First Hospital of Jilin University, ²The Skaggs School of Pharmacy and Pharmaceutical Sciences, Department of Pharmaceutical Sciences, University of Colorado Anschutz Medical Campus, ³Key Laboratory of Zoonoses Research, Ministry of Education, Jilin University, ⁴Department of Cardiology, China-Japan Union Hospital of Jilin University, ⁵Department of Gastrointestinal, Colorectal and Anal Surgery, China-Japan Union Hospital of Jilin University

JoVE 60847

Setup and Execution of the Rapid Cycle Deliberate Practice Death Notification Curriculum

Patrick G. Hughes¹, Kate E. Hughes², Mary J. Hughes^3,4, Lindsay Weaver⁴, Lauren E. Falvo⁴, Anna M. Bona⁴, Dylan Cooper⁴, Cherri Hobgood⁴, Rami A. Ahmed⁴

¹Florida Atlantic University Schmidt College of Medicine, ²Department of Emergency Medicine, University of Arizona College of Medicine, ³Department of Osteopathic Medical Specialties, College of Osteopathic Medicine, Michigan State University, ⁴Indiana University School of Medicine

JoVE 61646

3D Planning and Printing of Patient Specific Implants for Reconstruction of Bony Defects

Tal Capucha*¹, Dekel Shilo*^1,2, Ori Blanc¹, Shahar Turgeman¹, Omri Emodi^1,2, Adi Rachmiel^1,2

¹Department of Oral and Maxillofacial Surgery, Rambam Medical Care Center, ²Ruth & Bruce Rappaport Faculty of Medicine, Technion-Israel Institute of Technology

JoVE 60929

Applying a Three-dimensional Uniaxial Mechanical Stimulation Bioreactor System to Induce Tenogenic Differentiation of Tendon-Derived Stem Cells

Ziming Chen*¹, Peilin Chen*¹, Rui Ruan*¹, Lianzhi Chen¹, Jun Yuan¹, David Wood¹, Tao Wang¹, Ming Hao Zheng¹

¹Centre of Orthopaedic Translational Research, Medical School, University of Western Australia

JoVE 61278

Dynamic Measurement and Imaging of Capillaries, Arterioles, and Pericytes in Mouse Heart

Guiling Zhao¹, Humberto C. Joca¹, W. Jonathan Lederer¹

¹Laboratory of Molecular Cardiology, Center for Biomedical Engineering and Technology and Department of Physiology, University of Maryland School of Medicine

JoVE 61566

A Novel Right Ventricular Volume and Pressure Loaded Piglet Heart Model for the Study of Tricuspid Valve Function.

Lily Q. Lin¹, Sanaz Hatami², James Yashu Coe¹, Timothy M. Colen¹, Consolato Sergi³, Richard Thompson⁴, Elena S. Di Martino⁵, Walter Herzog⁶, Ziad Abu Sara⁶, Darren H. Freed², Nee Scze Khoo¹

¹Division of Pediatric Cardiology, Department of Pediatrics, University of Alberta, ²Department of Surgery, University of Alberta, ³Department of Laboratory Medicine and Pathology, University of Alberta, ⁴Department of Biomedical Engineering, University of Alberta, ⁵Department of Civil Engineering, University of Calgary, ⁶Faculty of Kinesiology, University of Calgary

JoVE 61251

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