Raymond J. Owens Oxford Protein Production Facility Research Complex at Harwell Biography Publications Institution JoVE Articles Raymond J. Owens has not added a biography. If you are Raymond J. Owens and would like to personalize this page please email our Author Liaison for assistance. Publications GFP-Based Expression Screening of Membrane Proteins in Insect Cells Using the Baculovirus System Methods in Molecular Biology (Clifton, N.J.). 2015 | Pubmed ID: 25502201 Transient Expression in HEK 293 Cells: An Alternative to E. Coli for the Production of Secreted and Intracellular Mammalian Proteins Methods in Molecular Biology (Clifton, N.J.). 2015 | Pubmed ID: 25447866 Adhiron: a Stable and Versatile Peptide Display Scaffold for Molecular Recognition Applications Protein Engineering, Design & Selection : PEDS. May, 2014 | Pubmed ID: 24668773 Application of In-Fusion™ Cloning for the Parallel Construction of E. Coli Expression Vectors Methods in Molecular Biology (Clifton, N.J.). 2014 | Pubmed ID: 24395367 Chromophore-linked Substrate (CLS405): Probing Metallo-β-lactamase Activity and Inhibition ChemMedChem. Dec, 2013 | Pubmed ID: 24166830 A Streamlined Implementation of the Glutamine Synthetase-based Protein Expression System BMC Biotechnology. 2013 | Pubmed ID: 24063773 Binding of (5S)-penicilloic Acid to Penicillin Binding Protein 3 ACS Chemical Biology. Oct, 2013 | Pubmed ID: 23899657 Assay Platform for Clinically Relevant Metallo-β-lactamases Journal of Medicinal Chemistry. Sep, 2013 | Pubmed ID: 23898798 Crystal Structure of Signal Regulatory Protein Gamma (SIRPγ) in Complex with an Antibody Fab Fragment BMC Structural Biology. 2013 | Pubmed ID: 23826770 S-Adenosyl-S-carboxymethyl-L-homocysteine: a Novel Cofactor Found in the Putative TRNA-modifying Enzyme CmoA Acta Crystallographica. Section D, Biological Crystallography. Jun, 2013 | Pubmed ID: 23695253 Expression of Recombinant Glycoproteins in Mammalian Cells: Towards an Integrative Approach to Structural Biology Current Opinion in Structural Biology. Jun, 2013 | Pubmed ID: 23623336 Structure of Ribose 5-phosphate Isomerase from the Probiotic Bacterium Lactobacillus Salivarius UCC118 Acta Crystallographica. Section F, Structural Biology and Crystallization Communications. Dec, 2012 | Pubmed ID: 23192019 Structural and Functional Characterization of the Kindlin-1 Pleckstrin Homology Domain The Journal of Biological Chemistry. Dec, 2012 | Pubmed ID: 23132860 Outrunning Free Radicals in Room-temperature Macromolecular Crystallography Acta Crystallographica. Section D, Biological Crystallography. Jul, 2012 | Pubmed ID: 22751666 Structure of the Regulatory Domain of the LysR Family Regulator NMB2055 (MetR-like Protein) from Neisseria Meningitidis Acta Crystallographica. Section F, Structural Biology and Crystallization Communications. Jul, 2012 | Pubmed ID: 22750853 In Situ Macromolecular Crystallography Using Microbeams Acta Crystallographica. Section D, Biological Crystallography. May, 2012 | Pubmed ID: 22525757 Converting Monoclonal Antibodies into Fab Fragments for Transient Expression in Mammalian Cells Methods in Molecular Biology (Clifton, N.J.). 2012 | Pubmed ID: 21987252 Streamlining Homogeneous Glycoprotein Production for Biophysical and Structural Applications by Targeted Cell Line Development PloS One. 2011 | Pubmed ID: 22174749 Structure of New Delhi Metallo-β-lactamase 1 (NDM-1) Acta Crystallographica. Section F, Structural Biology and Crystallization Communications. Oct, 2011 | Pubmed ID: 22102018 XtalPiMS: a PiMS-based Web Application for the Management and Monitoring of Crystallization Trials Journal of Structural Biology. Aug, 2011 | Pubmed ID: 21605683 Expression of Protein Complexes Using Multiple Escherichia Coli Protein Co-expression Systems: a Benchmarking Study Journal of Structural Biology. Aug, 2011 | Pubmed ID: 21382497 Crystal Structures of Penicillin-binding Protein 3 from Pseudomonas Aeruginosa: Comparison of Native and Antibiotic-bound Forms Journal of Molecular Biology. Jan, 2011 | Pubmed ID: 20974151 Novel Structural Features in Two ZHX Homeodomains Derived from a Systematic Study of Single and Multiple Domains BMC Structural Biology. 2010 | Pubmed ID: 20509910 Structural and Functional Insights of RANKL-RANK Interaction and Signaling Journal of Immunology (Baltimore, Md. : 1950). Jun, 2010 | Pubmed ID: 20483727 The Structure of a Reduced Form of OxyR from Neisseria Meningitidis BMC Structural Biology. 2010 | Pubmed ID: 20478059 Improved Expression of Secreted and Membrane-targeted Proteins in Insect Cells Biotechnology and Applied Biochemistry. Jul, 2010 | Pubmed ID: 20441568 The Crystal Structure of NGO0477 from Neisseria Gonorrhoeae Reveals a Novel Protein Fold Incorporating a Helix-turn-helix Motif Proteins. May, 2010 | Pubmed ID: 20196080 Recent Advances in the Production of Proteins in Insect and Mammalian Cells for Structural Biology Journal of Structural Biology. Oct, 2010 | Pubmed ID: 20153433 Structure and Functionality in Flavivirus NS-proteins: Perspectives for Drug Design Antiviral Research. Aug, 2010 | Pubmed ID: 19945487 Genetic Modification of a Baculovirus Vector for Increased Expression in Insect Cells Cell Biology and Toxicology. Feb, 2010 | Pubmed ID: 19655260 Structural Plasticity of Eph Receptor A4 Facilitates Cross-class Ephrin Signaling Structure (London, England : 1993). Oct, 2009 | Pubmed ID: 19836338 Crystal Structure of a Novel Conformational State of the Flavivirus NS3 Protein: Implications for Polyprotein Processing and Viral Replication Journal of Virology. Dec, 2009 | Pubmed ID: 19793813 Crystallization and Preliminary X-ray Analysis of Mouse RANK and Its Complex with RANKL Acta Crystallographica. Section F, Structural Biology and Crystallization Communications. Jun, 2009 | Pubmed ID: 19478440 The Structure of CrgA from Neisseria Meningitidis Reveals a New Octameric Assembly State for LysR Transcriptional Regulators Nucleic Acids Research. Aug, 2009 | Pubmed ID: 19474343 The Structure of NMB1585, a MarR-family Regulator from Neisseria Meningitidis Acta Crystallographica. Section F, Structural Biology and Crystallization Communications. Mar, 2009 | Pubmed ID: 19255465 The Production of Glycoproteins by Transient Expression in Mammalian Cells Methods in Molecular Biology (Clifton, N.J.). 2009 | Pubmed ID: 18988030 The Precise Engineering of Expression Vectors Using High-throughput In-Fusion PCR Cloning Methods in Molecular Biology (Clifton, N.J.). 2009 | Pubmed ID: 18988019 Rhabdovirus Matrix Protein Structures Reveal a Novel Mode of Self-association PLoS Pathogens. Dec, 2008 | Pubmed ID: 19112510 Generation of Baculovirus Vectors for the High-throughput Production of Proteins in Insect Cells Biotechnology and Bioengineering. Dec, 2008 | Pubmed ID: 18781697 Crystallization and Preliminary X-ray Analysis of CrgA, a LysR-type Transcriptional Regulator from Pathogenic Neisseria Meningitidis MC58 Acta Crystallographica. Section F, Structural Biology and Crystallization Communications. Sep, 2008 | Pubmed ID: 18765907 Vaccinia Virus Proteins A52 and B14 Share a Bcl-2-like Fold but Have Evolved to Inhibit NF-kappaB Rather Than Apoptosis PLoS Pathogens. 2008 | Pubmed ID: 18704168 A Pipeline for the Production of Antibody Fragments for Structural Studies Using Transient Expression in HEK 293T Cells Protein Expression and Purification. Nov, 2008 | Pubmed ID: 18662785 The Crystal Structure of UMP Kinase from Bacillus Anthracis (BA1797) Reveals an Allosteric Nucleotide-binding Site Journal of Molecular Biology. Sep, 2008 | Pubmed ID: 18625239 Structures of an Alanine Racemase from Bacillus Anthracis (BA0252) in the Presence and Absence of (R)-1-aminoethylphosphonic Acid (L-Ala-P) Acta Crystallographica. Section F, Structural Biology and Crystallization Communications. May, 2008 | Pubmed ID: 18453697 Expression, Purification and Crystallization of a Lyssavirus Matrix (M) Protein Acta Crystallographica. Section F, Structural Biology and Crystallization Communications. Apr, 2008 | Pubmed ID: 18391421 Structure of the Cold-shock Domain Protein from Neisseria Meningitidis Reveals a Strand-exchanged Dimer Acta Crystallographica. Section F, Structural Biology and Crystallization Communications. Apr, 2008 | Pubmed ID: 18391418 Protein Production and Purification Nature Methods. Feb, 2008 | Pubmed ID: 18235434 Semi-automated Microseeding of Nanolitre Crystallization Experiments Acta Crystallographica. Section F, Structural Biology and Crystallization Communications. Jan, 2008 | Pubmed ID: 18097093 Structure of the Murray Valley Encephalitis Virus RNA Helicase at 1.9 Angstrom Resolution Protein Science : a Publication of the Protein Society. Oct, 2007 | Pubmed ID: 17893366 Crystal Structure of the Murray Valley Encephalitis Virus NS5 Methyltransferase Domain in Complex with Cap Analogues The Journal of General Virology. Aug, 2007 | Pubmed ID: 17622627 The Structure and Transcriptional Analysis of a Global Regulator from Neisseria Meningitidis The Journal of Biological Chemistry. May, 2007 | Pubmed ID: 17374605 Glycoprotein Structural Genomics: Solving the Glycosylation Problem Structure (London, England : 1993). Mar, 2007 | Pubmed ID: 17355862 A Versatile Ligation-independent Cloning Method Suitable for High-throughput Expression Screening Applications Nucleic Acids Research. 2007 | Pubmed ID: 17317681 Analysis of Variable N-glycosylation Site Occupancy in Glycoproteins by Liquid Chromatography Electrospray Ionization Mass Spectrometry Analytical Biochemistry. Feb, 2007 | Pubmed ID: 17178093 Lysine Methylation As a Routine Rescue Strategy for Protein Crystallization Structure (London, England : 1993). Nov, 2006 | Pubmed ID: 17098187 Implementation of Semi-automated Cloning and Prokaryotic Expression Screening: the Impact of SPINE Acta Crystallographica. Section D, Biological Crystallography. Oct, 2006 | Pubmed ID: 17001088 Structure of the PII Signal Transduction Protein of Neisseria Meningitidis at 1.85 A Resolution Acta Crystallographica. Section F, Structural Biology and Crystallization Communications. Jun, 2006 | Pubmed ID: 16754965 Crystal Structure of Nitrogen Regulatory Protein IIANtr from Neisseria Meningitidis BMC Structural Biology. 2005 | Pubmed ID: 16092953 Verde Fluorescente à base de proteínas Expressão Rastreio de Proteínas de Membrana em Louise E. Bird1, Heather Rada1, Anil Verma1, Raphael Gasper2,3, James Birch4, Matthew Jennions4, Jan Lӧwe3, Isabel Moraes4, Raymond J. Owens1 1Oxford Protein Production Facility, Research Complex at Harwell, 2Protein Crystallography Group, Ruhr University, 3MRC Laboratory of Molecular Biology, Cambridge Biomedical Campus, 4Membrane Protein Laboratory, Diamond Light Source JoVE 52357 생물학
Verde Fluorescente à base de proteínas Expressão Rastreio de Proteínas de Membrana em Louise E. Bird1, Heather Rada1, Anil Verma1, Raphael Gasper2,3, James Birch4, Matthew Jennions4, Jan Lӧwe3, Isabel Moraes4, Raymond J. Owens1 1Oxford Protein Production Facility, Research Complex at Harwell, 2Protein Crystallography Group, Ruhr University, 3MRC Laboratory of Molecular Biology, Cambridge Biomedical Campus, 4Membrane Protein Laboratory, Diamond Light Source JoVE 52357 생물학