Microperfusion Assessment
Microperfusion Assessment - Related Videos
08:50
Use of Cerebral Open-Flow Microperfusion for the Longitudinal Collection of Interstitial Fluid in an Animal Model of Glioblastoma
0 Views •
2025
In vivo, intercellular communications underpinning many central nervous system diseases, such as glioblastoma (GBM), are notoriously difficult to measure and characterize. Here, we describe procedures of cerebral open-flow microperfusion (cOFM) that can be employed to sample interstitial fluid components in a longitudinal animal model of GBM.
10:27
Real-Time Assessment of Spinal Cord Microperfusion in a Porcine Model of Ischemia/Reperfusion
0 Views •
Cited by 5 •
2020
Spinal cord microcirculation plays a pivotal role in spinal cord injury. Most methods do not allow real-time assessment of spinal cord microcirculation, which is essential for the development of microcirculation-targeted therapies. Here, we propose a protocol using Laser-Doppler-Flow Needle probes in a large animal model of ischemia/reperfusion.
12:48
Microperfusion Technique to Investigate Regulation of Microvessel Permeability in Rat Mesentery
0 Views •
Cited by 3 •
2015
The modified Landis technique enables paired measurement of the hydraulic conductivity of individual microvessels in the mesentery of normal and genetically modified rats under control and test conditions using microperfusion techniques. It provides a convenient method to evaluate mechanisms that regulate microvessel permeability and transvascular exchange under physiological conditions.
08:36
Neurotoxicity Assessment in Adult Danio rerio using a Battery of Behavioral Tests in a Single Tank
0 Views •
Cited by 3 •
2023
Here, we present a comprehensive behavioral test battery, including the novel tank, Shoaling, and social preference tests, to effectively determine the potential neurotoxic effects of chemicals (e.g., methamphetamine and glyphosate) on adult zebrafish using a single tank. This method is relevant to neurotoxicity and environmental research.
06:50
Systematic Assessment of Well-Being in Mice for Procedures Using General Anesthesia
0 Views •
Cited by 25 •
2018
We developed a protocol to assess well-being in mice during procedures using general anesthesia. A series of behavioral parameters indicating levels of well-being as well as glucocorticoid metabolites were analyzed. The protocol can serve as a general aid to estimate the degree of severity in a scientific, animal-centered manner.
FAQs
The assessment combines controlled perfusion with measurements of flow rate, pressure, imaging, or tracer movement. Comparing these observations shows whether fluid travels through the intended channels and reaches relevant regions efficiently. It can also expose transport limitations or uneven delivery, allowing investigators to connect local flow behavior with potential effects on cell survival and function.
Tracer movement provides an observable indication of how fluid and dissolved material travel through small channels or across engineered regions. Patterns of movement can reveal restricted transport, incomplete delivery, or differences between locations within a construct. This information complements flow and pressure measurements by addressing exchange and distribution rather than only the imposed perfusion conditions.
Imaging can show where perfusion is distributed within a network, tissue, or engineered construct instead of reporting only an overall rate. Spatial patterns may identify nonuniform perfusion, poorly reached regions, or localized vessel dysfunction. These findings are important when the average flow appears acceptable but different areas may experience substantially different transport conditions.
A useful measurement set may include imposed or observed flow rate, pressure, images of fluid distribution, and tracer movement. Each measurement addresses a different aspect of microscale transport: flow and pressure describe perfusion conditions, while imaging and tracers help show spatial delivery and exchange. Combining them produces a more informative characterization than relying on one measurement alone.
The workflow begins by establishing controlled perfusion through the selected vascular network, tissue, or engineered construct. Investigators then record relevant flow or pressure measurements and may track distribution through imaging or tracer movement. The resulting data are examined for delivery efficiency, transport limitations, nonuniform perfusion, or signs of vessel dysfunction, depending on the system being studied.
Researchers apply them when evaluating engineered blood vessels, organ-on-chip systems, or tissue scaffolds whose performance depends on microscale fluid delivery. The results can guide device optimization, support disease modeling, and indicate whether a tissue-engineered system provides sufficiently functional transport. Microperfusion assessment therefore links physical flow behavior with biological performance during design and evaluation.