Lung Fixation
Lung Fixation - Related Videos
06:57
Instillation and Fixation Methods Useful in Mouse Lung Cancer Research
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Cited by 48 •
2015
The goal of this paper is to describe simple methods that will greatly aid in the setup and analysis of mouse lungs with lung cancer or other pathologies. We present 3 protocols to simply and reliably carry out lung instillations, fixation, and lung volume measurements.
05:35
Lung Instillation and Fixation: Introducing Liquid into the Trachea and Fixing Lungs to Measure the Fixed Lung Volume
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2023
This video describes a simple and reliable method of introducing liquid into the trachea for lung instillation, fixation, and lung volume measurement.
07:19
Air-Inflation of Murine Lungs with Vascular Perfusion-Fixation
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Cited by 3 •
2021
Presented is a method for air-inflation with vascular perfusion-fixation of the lungs that preserves the location of cells within airways, alveoli and interstitium for structure-function analyses. Constant airway pressure is maintained with an air-inflation chamber while fixative is perfused via the right ventricle. Lungs are processed for histologic studies.
05:48
Lung Fixation under Constant Pressure for Evaluation of Emphysema in Mice
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Cited by 4 •
2019
Presented here is a useful protocol for lung fixation that creates a stable condition for histological evaluate of lung specimens from a mouse model of emphysema. The main advantage of this model is that it can fix many lungs with the same constant pressure without lung collapse or deflation.
08:53
Whole Animal Perfusion Fixation for Rodents
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Cited by 531 •
2012
Here we describe a low-cost, rapid, controlled and uniform fixation procedure using 4% paraformaldehyde perfused via the vascular system: through the heart of the rat to obtain the best possible preservation of the brain.
FAQs
Formalin chemically cross-links proteins and other cellular components, stabilizing tissue features so they remain available for examination after processing. This chemical preservation helps maintain cellular morphology for histology and immunohistochemistry, while also supporting later assessment of disease-related changes. In cancer studies, the resulting stability allows investigators to compare tumor-associated features across preserved specimens.
These approaches help maintain the lung’s air-space structure while the tissue is being preserved and processed. Without attention to how the lung is expanded during fixation, architectural features may be less representative of their original arrangement. Preserving air spaces is particularly relevant when cancer researchers examine tumor development, tissue invasion, or other changes involving lung structure.
Preserved specimens can retain information about overall architecture, cellular features, and disease-related alterations. Those features support histological examination and immunohistochemistry, which can be used to study tissue organization and specific cellular characteristics. In preclinical lung cancer models, this enables assessment of how tumors develop within the lung and how invasive changes appear in preserved sections.
Consistent fixation reduces variation introduced during tissue preservation, making differences between specimens easier to interpret. When samples receive comparable treatment, researchers can more reliably compare tumor development, invasion, or treatment responses across experimental groups. This standardization strengthens the use of fixed lung tissue in preclinical studies and improves the comparability of findings between experiments.
After preservation, the tissue can be processed into sections for microscopic analysis. Researchers may apply histological stains to examine general structure, use immunohistochemistry to assess cellular features, or perform molecular assays to investigate additional disease-related information. Selecting among these downstream approaches allows the same fixed specimen type to support complementary analyses of lung cancer models.
Fixed lung tissue provides a stable basis for examining changes associated with treatment in preclinical cancer models. Researchers can compare preserved samples from different experimental conditions using histology, immunohistochemistry, or molecular assays. These analyses may reveal differences in tumor development, invasion, or other disease-related features, helping relate tissue-level findings to the effects of an intervention.
Lung fixation is useful when investigators need to examine preserved tissue architecture and cellular features in experimental lung cancer models. It supports analyses of tumor development and invasion, while enabling histological, immunohistochemical, and molecular assessment after processing. Because controlled preservation improves comparability, the approach is especially valuable for comparing experimental groups or evaluating responses to treatment.