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Identificatie en analyse van de muis erytroïde voorouders met behulp van de CD71/TER119 Flow-cytometric Assay

Article DOI: 10.3791/2809-v • 15:32 min • August 5th, 2011
August 5th, 2011 •
*1, *1, *1, *1, 1, 1
1Department of Pediatrics and Department of Cancer Biology, University of Massachusetts Medical School
* These authors contributed equally

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Een flow-cytometrische methode voor de identificatie en moleculaire analyse van differentiatie-fase-specifieke muriene erytroïde voorlopers en precursoren, direct in vers geoogste muis beenmerg, milt of foetale lever. De test is gebaseerd op het celoppervlak markers CD71, Ter119, en de celgrootte.

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Developmental Biology erytropoëse hematopoëtische voorlopercellen flow-cytometrie erytropoëtine EpoR-/ - muis erytropoëtische stress foetale erytropoëse CD71 Ter119 foetale lever erytroïd subsets erythroblast celcyclus
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