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In vitro Uncoating av HIV-1 kärnor

Article DOI: 10.3791/3384-v • 10:49 min • November 8th, 2011
November 8th, 2011 •
1, 1
1Department of Pathology, Microbiology and Immunology, Vanderbilt University School of Medicine

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Uncoating är ett viktigt steg i den tidiga fasen av HIV-1 livscykel och definieras som demonteringen av kapsid skalet och frisläppandet av den virala ribonucleoprotein komplexa (vRNP). Här visar vi tekniker för att isolera intakta kärnor från HIV-1 virioner och för att kvantifiera deras uncoating

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Immunologi 57 lentivirus hiv virus infektion kapsid 293T Celler
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