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Un procédé amélioré pour la préparation de collagène de type I de la peau

Article DOI: 10.3791/51011-v • 05:17 min • January 21st, 2014
January 21st, 2014 •
1,2, 1, 1,2
1Department of Anesthesiology, Perioperative and Pain Medicine, Boston Children's Hospital, 2Department of Anesthesia, Harvard Medical School

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Les procédures traditionnelles pour l'isolement de soluble de type 1 collagène (COL1) nécessitent environ 10 jours à partir de début à la fin en raison d'incubations de tampons de longues et laborieuses remises en suspension de fibrilles. Ici, nous décrivons un moyen de purifier COL1 de petites biopsies cutanées en moins de 3 heures.

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Bio-ingénierie Numéro 83 collagène de type 1 la matrice extracellulaire l'ingénierie tissulaire les protéines d'échafaudage derme corium
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