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Kombinert DNA-RNA Fluorescent

Article DOI: 10.3791/51628-v • 15:54 min • June 14th, 2014
June 14th, 2014 •
1, 1
1Department of Reproduction and Development, Erasmus MC - University Medical Center

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Fluorescerende in situ hybridisering (FISH) tillater påvisning av nukleinsyrer i sitt opprinnelige miljø i cellene. Vi beskriver her en protokoll for det kombinerte, samtidig påvisning av RNA-og DNA ved hjelp av FISH, som kan brukes til å studere X-kromosom inaktivering i mus embryonale stamceller.

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Biokjemi Fluorescent kombinert DNA-RNA FISH ES cell cytogenetikk enkelt celle analyse X kromosom inaktivering (XCI), Bacterial kunstig kromosom (BAC) DNA-probe,
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